Characterization of Native Bacillus Thuringiensis (Berliner) Isolates from India

被引：0
作者：
P. Anandhi
L. Saravanan
P. W. Ramtake
S. Elamathi
Sobita Simon
Savita Varma
机构：
[1] Allahabad Agricultural Institute,Department of Plant Protection
[2] Deemed University,Department of Biological Sciences
[3] Directorate of Oil Palm,Department of Agronomy
[4] Allahabad Agricultural Institute,undefined
[5] Deemed University,undefined
[6] Allahabad Agricultural Institute,undefined
[7] Deemed University,undefined
[8] Mahatma Gandhi Chitrakooot Gramodaya Vishwavidyalaya,undefined
来源：
National Academy Science Letters | 2012年 / 35卷
关键词：
Chemical characterization; Electron microscopy;
D O I：
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学科分类号：
摘要：
Five native isolates of Bacillus thuringiensis (B.t) were morphologically and biologically characterized. Gram staining, capsule staining, endospore staining and transmission electron microscopy confirmed the B.t. by morphologically. Poly-acrylamide-gel-electrophoresis showed that all the native isolates were producing 130–140 kDa proteins. Chemical characterization revealed that Isolates NSC-1, NSC-3 and COR-4 were fermented maltose while NSC-9 and GUR-5 showed delayed +ve reaction. All selected cultures were catalase +ve, ammonification −ve, citrate utilization +ve, casein hydrolysis +ve, gelatin hydrolysis −ve, indole production −ve and VP test −ve. In the bioassays, five native strains NSC-1, NSC-3, NSC-9, COR-4 and GUR-5 were identified and proved as a novel isolate for controlling Castor hairy caterpillar (Pericalia ricini, Arctidae; Lepidoptera).
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页码：243 / 247
页数：4
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