Gene Therapy Progress and Prospects: Electroporation and other physical methods

被引：0
作者：
D J Wells
机构：
[1] Gene Targeting Unit,Department of Cellular and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine
[2] Imperial College London,undefined
[3] Charing Cross Hospital,undefined
来源：
Gene Therapy | 2004年 / 11卷
关键词：
plasmid DNA; gene transfer; ultrasound; hydrodynamic; vascular; skeletal muscle;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Over the last 5 years, physical methods of plasmid delivery have revolutionized the efficiency of nonviral gene transfer, in some cases reaching the efficiencies of viral vectors. In vivo electroporation dramatically increases transfection efficiency for a variety of tissues. Other methods with clinical precedent, pressure-perfusion and ultrasound, also improve plasmid gene transfer. Alternatives such as focused laser, magnetic fields and ballistic (gene gun) approaches can also enhance delivery. As plasmid DNA appears to be a safe gene vector system, it seems likely that plasmid with physically enhanced delivery will be used increasingly in clinical trials.
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页码：1363 / 1369
页数：6
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