Tryptophan is a precursor for melatonin and serotonin biosynthesis in in vitro regenerated St. John's wort (Hypericum perforatum L. cv. Anthos) plants

被引：0

作者：

S. J. Murch

S. KrishnaRaj

P. K. Saxena

机构：

[1] Department of Plant Agriculture,

[2] Biotechnology Division,undefined

[3] Edmund C. Bovey Complex,undefined

[4] University of Guelph,undefined

[5] Guelph,undefined

[6] Ontario,undefined

[7] Canada,undefined

[8] N1G 2W1 Fax: +1-519-7670755 e-mail psaxena@uoguelph.ca,undefined

来源：

Plant Cell Reports | 2000年 / 19卷

关键词：

Tryptophan; Indoleacetic acid; Melatonin; Serotonin; St. John's wort;

D O I：

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中图分类号：

学科分类号：

摘要：

cv. Anthos) is presented. Isotope tracer experiments were performed on plantlets regenerated from thidiazuron-induced stem explants and grown on MS basal medium for 2 months. Radiolabel from 14C-tryptophan was recovered as 14C-indoleacetic acid, 14C-tryptamine, 14C-5-hydroxytryptophan, 14C-serotonin and 14C-melatonin in the treated St. John's wort plantlets. Chromatographic peak identity was confirmed by high performance liquid chromatography-mass spectrometry-mass spectrometry and quantification of melatonin by radioimmunoassay. Significantly more radiolabel was recovered in serotonin relative to melatonin under low light conditions with this ratio being reversed under increased lighting, indicating that the rate of flow through this biosynthetic pathway is regulated, at least in part, by light.

引用

页码：698 / 704

页数：6