Bacterial biosynthesis of a calcium phosphate bone-substitute material

被引:0
作者
Ania C. Thackray
Rachel L. Sammons
Lynne E. Macaskie
Ping Yong
Harriet Lugg
Peter M. Marquis
机构
[1] The University of Birmingham,Biomaterials Department, School of Dentistry
[2] The University of Birmingham,School of Biosciences
[3] Edgbaston,undefined
来源
Journal of Materials Science: Materials in Medicine | 2004年 / 15卷
关键词
Polyurethane; Calcium Phosphate; Serratia; Phosphate Layer; Phosphatase Enzyme;
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学科分类号
摘要
A species of Serratia bacteria produces nano-crystalline hydroxyapatite (HA) crystals by use of a cell-bound phosphatase enzyme, located both periplasmically and within extracellular polymeric materials. The enzyme functions in resting cells by cleaving glycerol-2-phosphate (G-2-P) to liberate free phosphate ions which combine with calcium in solution to produce a cell-bound calcium phosphate material. Bacteria grown as a biofilm on polyurethane reticulated foam cubes were challenged with calcium and G-2-P in a bioreactor to produce a 3-D porous bone-substitute material. The scaffold has 1 mm macropores and 1 μm micropores. XRD showed the crystallites to be 25–28 nm in size, resembling HA before sintering and β-tricalcium phosphate (β-TCP, whitlockite) after. When biofilm was grown on titanium discs and challenged with calcium and G-2-P, a calcium phosphate layer formed on the discs. Biomineralisation is therefore a potential route to production of precursor nanophase HA, which has the potential to improve strength. The scaffold material produced by this method could be used as a bone-filler or as an alternative method for coating implants with a layer of HA.
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页码:403 / 406
页数:3
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