Characterization of Potato spindle tuber viroid (PSTVd) incidence and new variants from ornamentals

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作者
Jaroslav Matoušek
Rajen J. J. Piernikarczyk
Petr Dědič
Josef Mertelík
Kateřina Uhlířová
Ganesh S. Duraisamy
Lidmila Orctová
Kateřina Kloudová
Jiří Ptáček
Gerhard Steger
机构
[1] BC ASCR v.v.i.,Institute of Physical Biology
[2] Institute of Plant Molecular Biology,undefined
[3] Potato Research Institute,undefined
[4] Silva Tarouca Research Institute for Landscape and Ornamental Gardening,undefined
[5] Heinrich-Heine-Universität Düsseldorf,undefined
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关键词
Viroid pathogenicity; Real-time PCR; Biolistic inoculation of plants;
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摘要
We analyzed the spreading and persistence of PSTVd variants in several ornamentals in the territory of the Czech Republic. The pool of PSTVd variants detected in Solanum jasminoides, S. muricatum, Datura sp. and Brugmansia sp. was biolistically transferred to Matricaria chamomilla, Argyranthemum frutescens and Diascia sp., species which we found as sensitive hosts for PSTVd from ornamentals. The PSTVd pool showed sequence changes and increased variation after its transfer to potato, suggesting a wide adaptation potential of PSTVd in this crop. Potato exhibited genotype-dependent leaf and spindle tuber symptoms, when inoculated with the sap from S. jasminoides infected with the predominant and sequence-stable PSTVd-S1.
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页码:93 / 101
页数:8
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