Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications

被引:0
作者
Kabkaew L. Sukontason
Pongruk Sribanditmongkol
Tarinee Chaiwong
Roy C. Vogtsberger
Somsak Piangjai
Kom Sukontason
机构
[1] Chiang Mai University,Department of Parasitology, Faculty of Medicine
[2] Chiang Mai University,Department of Forensic Medicine, Faculty of Medicine
[3] Midwestern State University,Department of Biology
来源
Parasitology Research | 2008年 / 103卷
关键词
Myiasis; Cephalic Region; Forensic Entomology; Caudal Segment; Posterior Spiracle;
D O I
暂无
中图分类号
学科分类号
摘要
In forensic investigations, all immature stages of flies (egg, larvae, and puparium) can serve as entomological evidence at death scenes. These insects are primarily used to estimate the post mortem interval (PMI), but can also be involved in the analysis of toxic substances, determining manner of death, and in indicating relocation of a corpse in homicide cases. In this study, we present the morphology of the egg, larvae, and puparium of Hemipyrellia ligurriens, a blow fly species of forensic importance in Thailand. Examination was conducted using scanning electron microscopy (SEM). The egg stage was found to display a relatively wide plastron region (or median hatch line area) that spans almost the entire length of the egg. The median hatch line is oriented in an upright position. External chorionic sculpture of the egg is present in a hexagonal pattern whose reticular boundaries are slightly elevated. In the larval stages, the most prominent morphological changes were detected upon comparison of the first to the second instar; whereas, the differences between second and third instar larvae were less obvious outside of the increase in number of posterior spiracular slits. Most of the major differences involve body size and structure of the anterior and posterior spiracles. Each anterior spiracle in both the second and third instars projects five to seven papillae apically. Each posterior spiracular disc of a third instar exhibits a complete peritreme, three spiracular slits, and a prominent button that is ventromedially located. The puparium is coarctate and features a clustered bubble membrane comprised of ≈57 mammillate structures positioned dorsolaterally on each side of the first abdominal segment in young puparia. This feature is replaced by short, tubular respiratory horns in aged puparia. This study provides more detailed exposure of important morphological features that can be used for accurate identification of immature stages of H. ligurriens. Information presented can aid in forensic investigations involving this fly species.
引用
收藏
页码:877 / 887
页数:10
相关论文
共 50 条
[41]   Breeding of the Scuttle Fly Megaselia scalaris in a fish Carcass and Implications for the use in Forensic Entomology (Diptera: Phoridae). [J].
Moretti, Thiago de Carvalho ;
Thyssen, Patricia Jacqueline ;
Solis, Daniel Russ .
ENTOMOLOGIA GENERALIS, 2009, 31 (04) :349-353
[42]   Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) development by landmark-based geometric morphometrics of cephalopharyngeal skeleton: a preliminary assessment for forensic entomology application [J].
Li-Xuan Sim ;
Raja M. Zuha .
Egyptian Journal of Forensic Sciences, 9
[43]   Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) development by landmark-based geometric morphometrics of cephalopharyngeal skeleton: a preliminary assessment for forensic entomology application [J].
Sim, Li-Xuan ;
Zuha, Raja M. .
EGYPTIAN JOURNAL OF FORENSIC SCIENCES, 2019, 9 (01)
[44]   Description of Immature Stages and Development Time of Paralucilia paraensis (Mello) (Diptera: Calliphoridae) Associated with the Decomposition of a Partially Submerged Swine Carcass [J].
Sales, T. ;
Ferreira-Keppler, R. L. ;
Oliveira-da-Silva, A. ;
Souza, A. S. B. .
NEOTROPICAL ENTOMOLOGY, 2013, 42 (02) :211-215
[45]   Description of Immature Stages and Development Time of Paralucilia paraensis (Mello) (Diptera: Calliphoridae) Associated with the Decomposition of a Partially Submerged Swine Carcass [J].
T Sales ;
R L Ferreira-Keppler ;
A Oliveira-da-Silva ;
A S B Souza .
Neotropical Entomology, 2013, 42 :211-215
[46]   Description of immature stages of the flesh fly Peckia (Sarcodexia) lambens (Wiedemann) (Diptera: Sarcophagidae) provides better resolution for taxonomy and forensics [J].
Vairo, K. P. ;
Queiroz, M. M. C. ;
Mendonca, P. M. ;
Barbosa, R. R. ;
de Carvalho, C. J. B. .
TROPICAL ZOOLOGY, 2015, 28 (03) :114-125
[47]   ON THE MORPHOLOGY AND MITOCHONDRIAL DNA BARCODING OF THE FLESH FLY SARCOPHAGA (LIOPYGIA) ARGYROSTOMA (ROBINEAU-DESVOIDY, 1830) (DIPTERA: SARCOPHAGIDAE) - AN IMPORTANT SPECIES IN FORENSIC ENTOMOLOGY [J].
Draber-Monko, Agnieszka ;
Malewski, Tadeusz ;
Pomorski, Jan ;
Los, Marta ;
Slipinski, Piotr .
ANNALES ZOOLOGICI, 2009, 59 (04) :465-493
[48]   A forensic toolkit for the "cheese skipper": Larval identification and thermal requirements of the immature stages of Piophila casei (L.) (Diptera: Piophilidae) [J].
Martin-Vega, Daniel ;
Beringola, Maria Luisa ;
Pedreno, Aaron .
FORENSIC SCIENCE INTERNATIONAL, 2025, 374
[49]   Ultrastructure analysis of the immature stages of Ravinia belforti (Diptera: Sarcophagidae), a species of medical-veterinary and forensic importance, by scanning electron microscopy [J].
da-Silva-Xavier, Alexandre ;
de Carvalho Queiroz, Margareth Maria .
ACTA TROPICA, 2016, 159 :192-199
[50]   Forensic veterinary use of the fly Lucilia sericata (Diptera: Calliphoridae) in the aspect of determining the time of death using tissues treated with calcium diclofenac [J].
Czepiel-Mil, Katarzyna ;
Listos, Piotr ;
Stryjecki, Robert ;
Kowalczyk-Pecka, Danuta ;
Nieoczym, Marek .
MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE, 2023, 79 (04) :172-176