Intrastriatal gene transfer of vascular endothelial growth factor rescues dopaminergic neurons in a rat Parkinson's disease model

被引：3

作者：

Tian Y. ^{[1
]}

Sun S. ^{[1
]}

Tang C. ^{[1
]}

Wang J. ^{[1
]}

Chen X. ^{[1
]}

Qiao X. ^{[1
]}

机构：

[1] Department of Neurology, Union Hospital, Tongji Medical College

来源：

Journal of Huazhong University of Science and Technology | 2006年 / 26卷 / 6期

关键词：

vascular endothelial growth factor A; Parkinson’s disease; gene therapy; gene transfer technique;

D O I：

10.1007/s11596-006-0611-7

中图分类号：

学科分类号：

摘要：

To examine the ability of intrastriatal gene transfer of vascular endothelial growth factor 165 mediated by adenoviral vector to rescue dopaminergic neurons in a rat model of Parkinson's disease (PD), we constructed recombinant replication-deficient adenoviral vectors carrying the gene of VEGF165 (Ad-VEGF), and injected Ad-VEGF (or Ad-LacZ and PBS as controls) into the striatum of rats 7 days after the lesion by 6-hydroxydopamine. The rat rotational behavior analysis and tyrosine hydroxylase (TH) immunohistochemistry were performed to assess the change of dopaminergic neurons. Our results showed that the rats receiving Ad-VEGF injection displayed a significant improvement in apomorphine-induced rotational behavior and a significant preservation of TH-positive neurons and fibers compared with control animals. It is concluded that intrastriatal gene transfer by Ad-VEGF may rescue the dopaminergic neurons from degeneration in a rat model of PD.

引用

页码：670 / 673

页数：3

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