Asymmetrical flow field-flow fractionation of white wine chromophoric colloidal matter

被引：0
作者：
Christian Coelho
Jérémie Parot
Michael Gonsior
Maria Nikolantonaki
Philippe Schmitt-Kopplin
Edith Parlanti
Régis D. Gougeon
机构：
[1] UMR PAM Université de Bourgogne/AgroSupDijon,Research Unit Analytical BioGeoChemistry, Department of Environmental Sciences
[2] Institut Universitaire de la Vigne et du Vin,Chair of Analytical Food Chemistry
[3] Jules Guyot,undefined
[4] Université de Bordeaux,undefined
[5] UMR 5805,undefined
[6] EPOC,undefined
[7] LPTC,undefined
[8] CNRS,undefined
[9] UMR 5805,undefined
[10] EPOC,undefined
[11] LPTC,undefined
[12] University of Maryland Center for Environmental Science,undefined
[13] Chesapeake Biological Laboratory,undefined
[14] Helmholtz Zentrum München,undefined
[15] Technische Universität München,undefined
来源：
Analytical and Bioanalytical Chemistry | 2017年 / 409卷
关键词：
Asymmetrical flow field-flow fractionation; White wine; Chromophoric colloidal matter; Wine proteins;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two analytical separation methods—size-exclusion chromatography and asymmetrical flow field-flow fractionation—were implemented to evaluate the integrity of the colloidal composition of Chardonnay white wine and the impact of pressing and fermentations on the final macromolecular composition. Wine chromophoric colloidal matter, representing UV–visible-absorbing wine macromolecules, was evaluated by optical and structural measurements combined with the description of elution profiles obtained by both separative techniques. The objective of this study was to apply these two types of fractionation on a typical Chardonnay white wine produced in Burgundy and to evaluate how each of them impacted the determination of the macromolecular chromophoric content of wine. UV–visible and fluorescence measurements of collected fractions were successfully applied. An additional proteomic study revealed that grape and microorganism proteins largely impacted the composition of chromophoric colloidal matter of Chardonnay wines. Asymmetrical flow field-flow fractionation appeared to be more reliable and less invasive with respect to the native chemical environment of chromophoric wine macromolecules, and hence is recommended as a tool to fractionate chromophoric colloidal matter in white wines.
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页码：2757 / 2766
页数：9
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共 287 条
  • [1] Vidal S(2003)The polysaccharides of red wine: total fractionation and characterization Carbohydr Polym 54 439-47
  • [2] Williams P(2001)Aggregation of grape seed tannins in model wine-effect of wine polysaccharides Food Hydrocoll 16 17-23
  • [3] Doco T(2006)Structure and properties of wine pigments and tannins Am J Enol Vitic 57 298-305
  • [4] Moutounet M(1998)Flavor-matrix interactions in wine ACS Symp Ser 714 217-29
  • [5] Pellerin P(2006)Protein haze in bottled white wines: how well do stability tests and bentonite fining trials predict haze formation during storage and transport? Aust J Grape Wine Res 12 212-20
  • [6] Riou V(2011)The effect of bentonite fining at different stages of white winemaking on protein stability Aust J Grape Wine Res 17 280-4
  • [7] Vernhet A(2010)Roles of grape thaumatin-like protein and chitinase in white wine haze formation J Agric Food Chem 59 733-40
  • [8] Doco T(2000)Thaumatin-like proteins and chitinases, the haze-forming proteins of wine, accumulate during ripening of grape (Vitis vinifera) berries and drought stress does not affect the final levels per berry at maturity J Agric Food Chem 48 1637-43
  • [9] Moutounet M(1999)Induction of different pathogenesis-related cDNAs in grapevine infected with powdery mildew and treated with ethephon Plant Pathol 48 325-36
  • [10] Cheynier V(1990)Fractionnement et caractérisation des glycoprotéines dans les moûts de raisin blancs J Int Sci Vigne Vin 24 13-28
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