Nitrite reductase genes as functional markers to investigate diversity of denitrifying bacteria during agricultural waste composting

被引:0
作者
Yaoning Chen
Wei Zhou
Yuanping Li
Jiachao Zhang
Guangming Zeng
Aizhi Huang
Jingxia Huang
机构
[1] Hunan University,College of Environmental Science and Engineering
[2] Key Laboratory of Environmental Biology and Pollution Control (Hunan University),College of Chemistry and Environment Engineering
[3] Ministry of Education,College of Resources and Environment
[4] Hunan City University,undefined
[5] Hunan Agricultural University,undefined
来源
Applied Microbiology and Biotechnology | 2014年 / 98卷
关键词
Composting; PCR-DGGE; Denitrifying bacteria; RDA; Phylogenetic analysis;
D O I
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中图分类号
学科分类号
摘要
The purpose of this study was to investigate the diversity of denitrifier community during agricultural waste composting. The diversity and dynamics of the denitrifying genes (nirK and nirS) were determined using polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE). Relationships between physico-chemical parameters and denitrifying genes structures were simultaneously evaluated by redundancy analysis (RDA). Phylogenetic analysis indicated that nirK clones grouped into six clusters and nirS clones into two major clusters, respectively. The results showed a very high diversity of nir gene sequences within composting samples. RDA showed that the nirK and nirS gene structures were significantly related to pH and pile temperature (P < 0.05). Significant amounts of the variation (49.2 and 38.3 % for nirK and nirS genes, respectively) were explained by pH and pile temperature, suggesting that those two parameters were the most likely ones to influence, or be influenced by the denitrifiers harboring nirK and nirS genes.
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页码:4233 / 4243
页数:10
相关论文
共 198 条
[1]  
Borcard D(1992)Partialling out the spatial component of ecological variation Ecology 73 1045-1055
[2]  
Legendre P(2000)Nitrite reductase genes ( Appl Environ Microbiol 66 2096-2104
[3]  
Drapeau P(2007) and Appl Environ Microbiol 73 6876-6884
[4]  
Braker G(2010)), as functional markers to investigate diversity of denitrifying bacteria in Pacific northwest marine sediment communities Micro Eco 60 850-861
[5]  
Zhou J(2010)Impact of plant functional group, plant species, and sampling time on the composition of Appl Environ Microbiol 76 1870-1878
[6]  
Wu L(2009)-type denitrifier communities in soil China. Microb Ecol 58 161-169
[7]  
Devol AH(2001)Impact of long-term fertilization on the composition of denitrifier communities based on nitrite reductase analyses in a paddy soil Soil Biol Biochem 33 853-866
[8]  
Tiedje JM(2005)Insights into the effect of soil pH on N Appl Environ Microbiol 71 8335-8343
[9]  
Bremer C(2003)O and N Bioresour Technol 89 109-114
[10]  
Braker G(2011) emissions and denitrifier community size and activity Environ Pollut 47 3105-3113