A strategy for co-translational folding studies of ribosome-bound nascent chain complexes using NMR spectroscopy
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作者:
Anaïs M E Cassaignau
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机构:Institute of Structu,
Anaïs M E Cassaignau
Hélène M M Launay
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机构:Institute of Structu,
Hélène M M Launay
Maria-Evangelia Karyadi
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机构:Institute of Structu,
Maria-Evangelia Karyadi
Xiaolin Wang
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机构:Institute of Structu,
Xiaolin Wang
Christopher A Waudby
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机构:Institute of Structu,
Christopher A Waudby
Annika Deckert
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Annika Deckert
Amy L Robertson
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机构:Institute of Structu,
Amy L Robertson
John Christodoulou
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机构:Institute of Structu,
John Christodoulou
Lisa D Cabrita
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机构:Institute of Structu,
Lisa D Cabrita
机构:
[1] Institute of Structu,
[2] ral and Molecular Biology,undefined
[3] University College London and Birkbeck College,undefined
[4] University of London,undefined
来源:
Nature Protocols
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2016年
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11卷
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摘要:
Cassaignau et al. provide a strategy for large-scale production and analysis of translationally arrested, isotopically labelled ribosome-bound nascent chains, enabling high-resolution co-translational protein folding studies using NMR spectroscopy.