The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme

被引:0
作者
Xinyi Wang
Yao Xu
Yang Chen
Limei Li
Feng Liu
Na Li
机构
[1] Peking University,Beijing National Laboratory for Molecular Sciences (BNLMS), The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering
[2] Shenyang Agricultural University,College of Sciences
来源
Analytical and Bioanalytical Chemistry | 2011年 / 400卷
关键词
Gold nanoparticles; Aggregation; Lysozyme; DNA aptamer; Plasmon resonance light scattering; Visual detection;
D O I
暂无
中图分类号
学科分类号
摘要
We developed a new simple and sensitive assay for lysozyme based on gold nanoparticle plasmon resonance light scattering (PRLS) measurement and naked-eye detection using for the first time the lysozyme DNA aptamer as the recognition element. Lysozyme DNA aptamer could stabilize gold nanoparticles (AuNPs) at high ionic strength. Introducing lysozyme to the system easily triggered the aggregation of AuNPs, producing a red-to-blue color change of the solution, red-shifted plasmon absorption, and enhanced plasmon resonance light scattering. The linear range was found to be 0.2∼4 nM for 0.7 nM AuNPs, 0.3∼6 nM for 1.4 nM AuNPs and 0.6∼8 nM for 2.1 nM AuNPs. About 0.1 nM lysozyme can produce an observable enhancement of PRLS signal. For visual detection, 1 nM lysozyme can produce a very distinctive color change. Satisfactory recoveries were obtained for simulated saliva and diluted urine samples, indicating that the method has potential for analyses of clinical samples. The simplicity and high sensitivity that are consistent with the resources and needs of many laboratories makes this method a good choice for routine analysis.
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页码:2085 / 2091
页数:6
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