Expressions of endothelin-1, fibronectin, and interleukin-1α of human umbilical vein endothelial cells under prolonged culture

被引：10

作者：

Kiyonaga H. ^{[1
]}

Doi Y. ^{[2
]}

Karasaki Y. ^{[3
]}

Arashidani K. ^{[4
]}

Itoh H. ^{[1
]}

Fujimoto S. ^{[2
]}

机构：

[1] Department of Surgery, University of Occupational and Environmental Health, School of Medicine, Kitakyushu 807-8555

[2] Department of Anatomy, University of Occupational and Environmental Health, School of Medicine, Kitakyushu

[3] Department of Medical Technology 1, University of Occupational and Environmental Health, School of Health Sciences, Kitakyushu

[4] Department of Environment Management, University of Occupational and Environmental Health, School of Health Sciences, Kitakyushu

来源：

Medical Electron Microscopy | 2001年 / 34卷 / 1期

关键词：

Endothelin-1; Fibronectin; HUVEC; Interleukin-1α; Weibel-Palade body;

D O I：

10.1007/s007950100003

中图分类号：

学科分类号：

摘要：

We examined human umbilical vein endothelial cells (HUVECs) under prolonged culture by electron microscopy and by light and electron immunocytochemistry including double immunolabeling. Based on the cell area of HUVECs through multiple passages, we divided the cells into first, second, and third stages, which exhibited distinct morphological and immunocytochemical characteristics. During the first stage, HUVECs were polygonal in shape and had already formed the monolayer confluence. During the second stage, they were characterized by an increased number of Weibel-Palade (WP) bodies, which were actively segregated from Golgi cisterns. Endothelin (ET)-1 and von Willebrand factor, an endothelial cell marker, were occasionally colocalized in WP bodies. The increase in WP bodies correlated with high ET-1 concentration in the cultured medium, suggesting that these inclusions are involved in storage and release of ET-1 in a manner indicating a regulatory pathway. During the third stage, fibronectin and interleukin (IL)-1α were expressed in HUVECs as well as in multinucleated giant cells, which originated from HUVECs, but WP bodies decreased in number in association with a decrease in ET-1 immunoreactivity and concentration. The foregoing changes in immunoreactivities for ET-1, fibronectin, and IL-1α affecting HUVECs under prolonged culture may reflect a senescent process of these cells.

引用

页码：41 / 53

页数：12

共 38 条

[21] Miyakawa M., Tsushima T., Isozaki O., Demura H., Shizume K., Arai M., Endothelin-1 stimulates c-fos mRNA expression and acts as a modulator on cell proliferation of rat FRTL5 thyroid cells, Biochem Biophys Res Commun, 184, pp. 231-238, (1992)
[22] Wagner D.D., Olmsted J.B., Marder V.J., Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells, J Cell Biol, 95, pp. 355-360, (1982)
[23] Ewenstein B.M., Warhol M.J., Handin R.I., Pober J.S., Composition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells, J Cell Biol, 104, pp. 1423-1433, (1987)
[24] Kagawa H., Fujimoto S., Electron-microscopic and immunocytochemical analyses of Weibel-Palade bodies in the human umbilical vein during pregnancy, Cell Tissue Res, 249, pp. 557-563, (1987)
[25] Sakamoto Y., Doi Y., Ohsato K., Fujimoto S., Immunoelectron microscopy on the localization of endothelin in the umbilical vein of perinatal rabbits, Anat Rec, 237, pp. 482-488, (1993)
[26] Doi Y., Ozaka T., Fukushige H., Furukawa H., Yoshizuka M., Fujimoto S., Increase in number of Weibel-Palade bodies and endothelin-1 release from endothelial cells in the cadmium-treated rat thoracic aorta, Virchows Arch, 428, pp. 367-373, (1996)
[27] Ozaka T., Doi Y., Kudo H., Fujimoto S., Enhancement in the synthesis and release of calcitonin gene-related peptide and endothelin-1 in the hypoxic rat carotid body artery, pp. 259-261, (1999)
[28] Kayashima K., Doi Y., Kudo H., Kiyonaga H., Fujimoto S., Effects of endothelin-1 on vasoactivity and its synthesis, storage, and acting sites in the rat superior mesenteric vasculature: An ultrastructural and immunocytochemical study, Med Electron Microsc, 32, pp. 36-42, (1993)
[29] Kumazaki T., Robetorye R.S., Robetorye S.C., Smith J.R., Fibronectin expression increases during in vitro cellular senescence: Correlation with increased cell area, Exp Cell Res, 195, pp. 13-19, (1991)
[30] Hara E., Nakada S., Takehana K., Nakajima T., Iino T., Oda K., Molecular cloning and characterization of cellular genes whose expression is repressed by the adenovirus E1a gene products and growth factors in quiescent rat cells, Gene (Amst), 70, pp. 97-106, (1988)

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