Real-time observation of the initiation of RNA polymerase II transcription

被引：0
|
作者：
Furqan M. Fazal
Cong A. Meng
Kenji Murakami
Roger D. Kornberg
Steven M. Block
机构：
[1] Stanford University,Department of Applied Physics
[2] Stanford University,Department of Chemistry
[3] Stanford University,Department of Structural Biology
[4] Stanford University,Department of Biology
[5] † Present address: Department of Biochemistry and Molecular Biophysics at the Perelman School of Medicine,undefined
[6] University of Pennsylvania,undefined
[7] Philadelphia,undefined
[8] Pennsylvania 19104,undefined
[9] USA.,undefined
来源：
Nature | 2015年 / 525卷
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摘要：
A single-molecule optical tweezer assay is developed to monitor transcription initiation in eukaryotic RNA polymerase II in real-time, making use of a highly purified preinitiation complex (PIC) from yeast; observations show that a large bubble is opened up in the DNA template during initiation, driven by the TFIIH helicase that forms part of the PIC, along with synthesis of an extended transcript before the transition from transcription initiation into elongation.
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页码：274 / 277
页数：3
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