The formation of a spheroid of primary hepatocytes and the expression of liver-specific functions depend on the characteristics of polyurethane foam

被引：0

作者：

Ijima H. ^{[1
]}

Nakazawa K. ^{[1
]}

Kaneko M. ^{[1
]}

Mizumoto H. ^{[1
]}

Matsushita T. ^{[1
]}

Gion T. ^{[2
]}

Shimada M. ^{[2
]}

Shirabe K. ^{[2
]}

Takenaka K. ^{[2
]}

Sugimachi K. ^{[2
]}

Funatsu K. ^{[1
]}

机构：

[1] Graduate School of Engineering, Kyushu University, 6-10-1 Hakozaki, Higashi-ku

[2] Department of Surgery II, Kyushu University, Fukuoka

来源：

Journal of Artificial Organs | 1998年 / 1卷 / 2期

关键词：

Contact angle; Hepatocytes spheroid; Polyurethane foam; Primary hepatocyte; Rapid spheroid formation;

D O I：

10.1007/BF02479990

中图分类号：

学科分类号：

摘要：

Rapid formation of spherical multicellular aggregates (spheroids) of hepatocytes and expression of liver-specific functions are important in developing a hybrid artificial liver. The relation between these points and the surface characteristics of the culture substratum are investigated in this article. Polyurethane foam (PUF) was used as a culture substratum for hepatocytes. Rat, dog, and porcine primary hepatocytes spontaneously formed a spheroid within 1.5 days in the pores of a hydrophilic PUF with a contact angle of 53.4 ± 2.7°. Rat and dog primary hepatocytes formed a spheroid within 3 and 7 days of culture, respectively, in hydrophobic PUF with a contact angle of about 100°. The rates of albumin secretion and ammonia metabolism at 5 days of culture of porcine hepatocytes were 31 μg of albumin/106 nuclei/day and 0.018 μmol of NH3/106 nuclei/h, respectively, about 2 times higher than the values with hydrophobic PUF. It is very important to control the hydrophilicity of the PUF surface to develop an effective artificial liver module. © The Japanese Society for Artificial Organs 1998.

引用

页码：83 / 88

页数：5

共 22 条

[21]

Lazar, A., Mann, H.J., Remmel, R.P., Shatford, R.A., Cerra, F.B., Hu, W.S., Extended liver-specific functions of porcine hepatocyte spheroids entrapped in collagen gel (1995) In Vitro Cell Dev Biol Anim, 31, pp. 340-346

[22]

Mizuno, S., Yanagi, K., Ohshima, N., Effects of surface modification of substrate material to attain highly concentrated hepatocytes culture (1990) Jpn J Artif Organs, 19, pp. 1161-1164

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