Metabolic engineering of Escherichia coli to produce (2S, 3R, 4S)-4-hydroxyisoleucine

被引：0

作者：

Sergey V. Smirnov

Tomohiro Kodera

Natalya N. Samsonova

Veronika А. Kotlyarovа

Natalya Yu Rushkevich

Аlexander D. Kivero

Pavel M. Sokolov

Makoto Hibi

Jun Ogawa

Sakayu Shimizu

机构：

[1] Ajinomoto-Genetika Research Institute,Division of Applied Life Sciences, Graduate School of Agriculture

[2] Institute of Life Sciences,undefined

[3] Ajinomoto Co.,undefined

[4] Inc.,undefined

[5] Kyoto University,undefined

来源：

Applied Microbiology and Biotechnology | 2010年 / 88卷

关键词：

4-Hydroxyisoleucine; Diabetes; Dioxygenase; Biotransformation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The stereo-specific l-isoleucine-4-hydroxylase (l-isoleucine dioxygenase (IDO)) was cloned and expressed in an Escherichia coli 2Δ strain lacking the activities of α-ketoglutarate dehydrogenase (EC 1.2.4.2), isocitrate liase (EC 4.1.3.1), and isocitrate dehydrogenase kinase/phosphatase (EC 2.7.11.5). The 2Δ strain could not grow in a minimal-salt/glucose/glycerol medium due to the blockage of TCA during succinate synthesis. The IDO activity in the 2Δ strain was able to “shunt” destroyed TCA, thereby coupling l-isoleucine hydroxylation and cell growth. Using this strain, we performed the direct biotransformation of l-isoleucine into 4-HIL with an 82% yield.

引用

页码：719 / 726

页数：7

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