Metabolic engineering of Escherichia coli to produce (2S, 3R, 4S)-4-hydroxyisoleucine

被引：0
|
作者：
Sergey V. Smirnov
Tomohiro Kodera
Natalya N. Samsonova
Veronika А. Kotlyarovа
Natalya Yu Rushkevich
Аlexander D. Kivero
Pavel M. Sokolov
Makoto Hibi
Jun Ogawa
Sakayu Shimizu
机构：
[1] Ajinomoto-Genetika Research Institute,Division of Applied Life Sciences, Graduate School of Agriculture
[2] Institute of Life Sciences,undefined
[3] Ajinomoto Co.,undefined
[4] Inc.,undefined
[5] Kyoto University,undefined
来源：
Applied Microbiology and Biotechnology | 2010年 / 88卷
关键词：
4-Hydroxyisoleucine; Diabetes; Dioxygenase; Biotransformation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The stereo-specific l-isoleucine-4-hydroxylase (l-isoleucine dioxygenase (IDO)) was cloned and expressed in an Escherichia coli 2Δ strain lacking the activities of α-ketoglutarate dehydrogenase (EC 1.2.4.2), isocitrate liase (EC 4.1.3.1), and isocitrate dehydrogenase kinase/phosphatase (EC 2.7.11.5). The 2Δ strain could not grow in a minimal-salt/glucose/glycerol medium due to the blockage of TCA during succinate synthesis. The IDO activity in the 2Δ strain was able to “shunt” destroyed TCA, thereby coupling l-isoleucine hydroxylation and cell growth. Using this strain, we performed the direct biotransformation of l-isoleucine into 4-HIL with an 82% yield.
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页码：719 / 726
页数：7
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