A new efficient expression system for Bacillus and its application to production of recombinant phytase

被引：0
|
作者：
Janne Kerovuo
Niklas von Weymarn
Mira Povelainen
Sanna Auer
Andrei Miasnikov
机构：
[1] Kantvik Research Centre,Danisco Cultor Innovation
[2] Helsinki University of Technology,Laboratory of Bioprocess Engineering
[3] Kantvik Research Centre,Danisco Cultor Innovation
来源：
Biotechnology Letters | 2000年 / 22卷
关键词：
fed-batch cultivation; phosphate-starvation; phytase; plasmid;
D O I：
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学科分类号：
摘要：
A new expression system was developed for Bacillus subtilis.This system uses a shuttle vector (B. subtilis– Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillusphytase – phytase activity at the end of cultivation reached 28.7 U ml−1.
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页码：1311 / 1317
页数：6
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