Development of a Colloidal Gold-Based Nanobioprobe for the Detection of Glycated Albumin

被引：4

作者：

Wangoo N. ^{[1
,2
]}

Swami A. ^{[1
,2
]}

Kaur S. ^{[3
]}

Bansal K. ^{[2
]}

Sharma R.K. ^{[3
]}

机构：

[1] Department of Applied Sciences, University Institute of Engineering & Technology (U.I.E.T.), Panjab University, Chandigarh

[2] Centre for Nanoscience and Nanotechnology (U.I.E.A.S.T), Panjab University, Chandigarh

[3] Department of Chemistry and Centre for Advanced Studies in Chemistry, Panjab University, Chandigarh

来源：

BioNanoScience | 2016年 / 6卷 / 02期

关键词：

Diabetes; Glycated albumin; Gold nanoparticles; Immunoassay;

D O I：

10.1007/s12668-016-0203-3

中图分类号：

学科分类号：

摘要：

A simple, inexpensive, and quick colorimetric immunoassay for the determination of diabetic biomarker (glycated albumin) using antibody labeled gold nanoparticles is reported. The sensitivity of the assay on the basis of absorbance measurements is excellent with a detection limit of 1 μg/ml with 40 nm sized gold nanoparticles. The assay is free of washing cycle and result can be read in minutes. Also, the method requires very low amount of the sample as compared to the conventional immunossays. Therefore, this method can be used for the simple, low cost, and rapid detection of diabetes marker, glycated albumin. © 2016, Springer Science+Business Media New York.

引用

页码：132 / 138

页数：6

共 18 条

[1]

Roohk H.V., Zaidi A.R., A review of glycated albumin as an intermediate glycation index for controlling diabetes, Journal of Diabetes Science and Technology, 2, 6, pp. 1114-1121, (2008)

[2]

Sako T., Mori A., Lee P., Takahashi T., Izawa T., Karasawa S., Et al., Diagnostic significance of serum glycated albumin in diabetic dogs, Journal of Veterinary Diagnostic Investigation, 20, 5, pp. 634-638, (2008)

[3]

Morita S., Kasayama S., Deguchi R., Hirai K., Mukai K., Utsu Y., Et al., Glycated albumin, rather than Hba1c, reflects diabetic retinopathy in patients with type 2 diabetes mellitus, Journal of Diabetes & Metabolism, 4, (2013)

[4]

Yu F., Persson B., Lofas S., Knoll W., Surface plasmon fluorescence immunoassay of free prostate-specific antigen in human plasma at the femtomolar level, Analytical Chemistry, 76, pp. 6765-6770, (2004)

[5]

Healy D.A., Hayes C.J., Leonard P., McKenna L., O'Kennedy R., Biosensor developments: application to prostate-specific antigen detection, Trends in Biotechnology, 25, pp. 125-131, (2007)

[6]

Das J., Aziz M.A., Yang H., A nanocatalyst-based assay for proteins: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle label, Journal of the American Chemical Society, 128, pp. 16022-16023, (2006)

[7]

Warnick G.R., Nauck M., Rifai N., Evolution of methods for measurement of HDL-cholesterol: from ultracentrifugation to homogeneous assays, Clinical Chemistry, 47, pp. 1579-1596, (2001)

[8]

Kokko T., Kokko L., Soukka T., Lovgren T., Homogeneous non-competitive bioaffinity assay based on fluorescence resonance energy transfer, Analytica Chimica Acta, 585, pp. 120-125, (2007)

[9]

Xu X., Georganopoulou D.G., Hill H.D., Mirkin C.A., Homogeneous detection of nucleic acids based upon the light scattering properties of silver-coated nanoparticle probes, Analytical Chemistry, 79, pp. 6650-6654, (2007)

[10]

Nikoobakht B., El-Sayed M., Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method, Chemistry of Materials, 15, pp. 1957-1962, (2003)

← 1 2 →