Genomic sequence of an infectious bursal disease virus isolate from Zambia: classical attenuated segment B reassortment in nature with existing very virulent segment A

被引：0
作者：
C. J. Kasanga
T. Yamaguchi
H. M. Munang’andu
K. Ohya
H. Fukushi
机构：
[1] Sokoine University of Agriculture,Department of Veterinary Microbiology and Parasitology, Molecular Biology and Biotechnology Section
[2] Tottori University,The Avian Zoonoses Center, Faculty of Agriculture
[3] University of Zambia,Department of Paraclinical Sciences, School of Veterinary Medicine
[4] Gifu University,Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences
来源：
Archives of Virology | 2013年 / 158卷
关键词：
Deduce Amino Acid Sequence; Vaccine Virus; Genome Segment; Nucleotide Sequence Identity; Infectious Bursal Disease Virus;
D O I：
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摘要：
We determined the complete nucleotide sequence of an infectious bursal disease (IBD) virus (IBDV) isolate (designated KZC-104) from a confirmed IBD outbreak in Lusaka in 2004. The genome consisted of 3,074 and 2,651 nucleotides in the coding regions of segments A and B, respectively. Alignment of both nucleotide and deduced amino acid sequences and phylogenetic analysis revealed that the genome segment A of KZC-104 was derived from a very virulent (VV) strain, whereas its segment B was derived from a classical attenuated strain. On BLAST search, the full-length segment A and B sequences showed 98 % nucleotide sequence identity to the VV strain D6948 and 99.8 % nucleotide sequence identity to the classical attenuated strain D78. This is a unique IBDV reassortant strain that has emerged in nature, involving segment B of a cell-culture-adapted attenuated vaccine.
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页码：685 / 689
页数：4
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