Genetic diversity of Pseudo-nitzschia brasiliana (Bacillariophyceae) from Malaysia

被引：0
作者：
Hong-Chang Lim
Po-Teen Lim
Suriyanti Nyun-Pau Su
Sing-Tung Teng
Chui-Pin Leaw
机构：
[1] Universiti Malaysia Sarawak,Faculty of Resource Science and Technology
[2] Universiti Malaysia Sarawak,Institute of Biodiversity and Environmental Conservation
来源：
Journal of Applied Phycology | 2012年 / 24卷
关键词：
Genetic diversity; ITS rDNA; Secondary structure; Minimum spanning network;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To clarify the genetic diversity of a potentially toxic pennate diatom, Pseudo-nitzschia brasiliana found in Malaysian waters, 30 strains of P. brasiliana were established into clonal culture since May 2008. The ultrastructure of these strains was examined for confirmation of species identification. The genetic marker, internal transcribed spacer (ITS) of nuclear-encoded ribosomal DNA was used to examine the genetic diversity of P. brasiliana isolated from different geographical localities. The ITS sequences of P. brasiliana were highly conserved in their secondary structures, with five helices in the first internal transcribe spacer (ITS1) and four universal helices in the second internal transcribe spacer (ITS2) with a pseudo-helix. No compensatory base change was observed among the strains examined. Genetic divergences among the Malaysian strains ranged from 0.07 to 0.54%. The present study revealed a high genetic homogeneity of Malaysian P. brasiliana strains.
引用
收藏
页码：1465 / 1475
页数：10
相关论文
共 218 条
[11]  
Paparazzo FE(2005)Life cycle, size reduction patterns, and ultrastructure of the pennate planktonic diatom Eur J Phycol 40 379-395
[12]  
Almandoz G(2004) (Bacillariophyceae) Biology & Environment: Proc Roy Irish Acad 104 55-74
[13]  
Ferreyra G(2009)First report of Protist 160 9-20
[14]  
Schloss I(2009) Lundholm, Moestrup and Hasle 2003, a new potentially toxic species from Turkish Coast Harmful Algae 8 225-232
[15]  
Dogliotti A(1998)Pennate diatom J Phycol 34 126-137
[16]  
Rupolo V(2005) as the primary source of domoic acid, a toxin in shellfish from eastern Prince Edward Island, Canada J Phycol 41 506-514
[17]  
Paparazzo F(2010) (Bacillariophyceae): a cosmopolitan diatom species? Mol Ecol Resourc 10 564-567
[18]  
Esteves J(2005)Lack of population genetic structuring in the marine planktonic diatom Bioinformatics 21 2108-2109
[19]  
Ferrario M(2004) (Bacillariophyceae) in a heterogeneous area in the Southern Bight of the North Sea Nucleic Acids Res 32 307-315
[20]  
Amato A(1999)Limits to gene flow in a cosmopolitan marine planktonic diatom Nucl Acids Symp Ser 41 95-98
12345678910