Production and Characterization of an Antifungal Compound from Pseudomonas protegens Strain W45

被引：5

作者：

Bajpai A. ^{[1
]}

Singh B. ^{[2
]}

Joshi S. ^{[3
]}

Johri B.N. ^{[1
]}

机构：

[1] Department of Biotechnology, Barkatullah University, Bhopal, 462026, M.P.

[2] Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana

[3] Department of Microbiology, University of Delhi South Campus, New Delhi

来源：

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | 2018年 / 88卷 / 3期

关键词：

Antifungal compound; Optimization; Pseudomonas protegens; Rhizobacteria; Sclerotinia sclerotiorum;

D O I：

10.1007/s40011-017-0844-1

中图分类号：

学科分类号：

摘要：

Pseudomonas protegens strain W45 recovered from rhizosphere of wheat possesses potential to produce an antifungal compound in the culture medium. Therefore, to enhance its production, statistical optimization of medium was employed. Peptone, glycerol and incubation period were identified as significant variables affecting its production. These variables were further optimized by response surface methodology that resulted in 38% enhancement in inhibition zone with optimal values of 2.5%, 1.49% and 48 h for peptone, glycerol and incubation period, respectively. PCR amplification by gene specific primers for phloroglucinol, pyrrolnitrin and pyoluteorin resulted in amplicon of 745, 719 and 773 bp respectively, confirming the presence of all three genes. Antifungal compound was purified by thin layer chromatography. Gas chromatography mass spectrometry analysis of the methanolic extract reveals the presence of pyrrole type antifungal molecule 3-(2-methylpropyl)-hexahydropyrrolo [1,2-a]pyrazine-1,4-dione (C11H18N2O2). The compound significantly inhibited the growth of Sclerotinia sclerotiorum. © 2017, The National Academy of Sciences, India.

引用

页码：1081 / 1089

页数：8

共 44 条

[1]

Thomashow L.S., Weller D.M., Current concepts in the use of introduced bacteria for biological disease control: mechanisms and antifungal metabolites, Plant–microbe interactions, pp. 187-235, (1995)

[2]

Bossis E., Lemanceau P., Latour X., Garden L., The taxonomy of Pseudomonas fluorescens and Pseudomonas putida: current status and need for revision, Agronomie, 20, pp. 51-63, (2000)

[3]

Dwivedi D., Johri B.N., Antifungals from fluorescent pseudomonads: biosynthesis and regulation, Curr Sci, 85, pp. 1693-1703, (2003)

[4]

Raaijmakers J.M., Vlami M., de Souza J.T., Antibiotic production by bacterial biocontrol agents, Antonie Van Leeuwenhoek J Microb, 81, 1-4, (2002)

[5]

Ramette A., Frapolli M., Fischer-Le Saux M., Gruffaz C., Meyer J.M., Defago G., Et al., Pseudomonas protegens sp. nov., widespread plant-protecting bacteria producing the biocontrol compounds 2,4-diacetylphloroglucinol and pyoluteorin, Syst Appl Microbiol, 34, 3, pp. 180-188, (2011)

[6]

Shanahan P.O., Sullivan D.J., Simpson P., Glennon J.D., O'Gara F., Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production, Appl Environ Microbiol, 58, pp. 353-358, (1992)

[7]

Duffy B.K., Defago G., Environmental factors modulating antifungal and siderophore biosynthesis by P. fluorescences biocontol strain, Appl Environ Microbiol, 65, pp. 2429-2438, (1999)

[8]

Wang Y., Fang X., An F., Wang G., Zhang X., Improvement of antifungal activity of Xenorhabdus bovienii by medium optimization using response surface methodology, Microbial Cell Factories, 10, (2011)

[9]

He L., Xu Y.Q., Zhang X.H., Medium factor optimization and fermentation kinetics for phenazine-1-carboxylic acid production by Pseudomonas sp M18G, Biotechnol Bioeng, 100, pp. 250-259, (2008)

[10]

Guo Z., Shen L., Ji Z., Wu W., Enhanced production of a novel cyclic hexapeptide antibiotic (NW-G01) by Streptomyces alboflavus 313 using response surface methodology, Int J Mol Sci, 13, pp. 5230-5241, (2012)

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