Production of 2-O-α-d-glucopyranosyl l-ascorbic acid using cyclodextrin glucanotransferase from Paenibacillus sp.

被引：0
作者：
Hong-Ki Jun
Kyung-Mi Bae
Sung-Koo Kim
机构：
[1] Pusan National University,Division of Biological Sciences
[2] Pukyung National University,Department of Biotechnology and Bioengineering
来源：
Biotechnology Letters | 2001年 / 23卷
关键词：
cyclodextrin glucanotransferase; 2-; -α-d-glucopyranosyl l-ascorbic acid; sp.; transglycosylation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Transglycosylation to produce a 2-O-α-d-glucopyranosyl l-ascorbic acid (AA-2G) was studied using cyclodextrin glucanotransferase (CGTase) from Paenibacillus sp. A series of maltooligosaccharides substituted 2-O-derivatives of l-ascorbic acid (AA) were analyzed by HPLC. The maltooligosaccharides were hydrolyzed by glucoamylase to give AA-2G. CGTase also produced AA-2G using dextrin as a glycosyl donor and AA as an acceptor. CGTase utilized α-, β-, and γ-CDs, amylose, soluble starch and corn starch as glycosyl donors but not glucose.
引用
收藏
页码：1793 / 1797
页数：4
相关论文
共 65 条
  • [1] Aga H(1991)Synthesis of 2- Agric. Biol. Chem. 55 1751-1756
  • [2] Yoneyama M(2001)- J. Microb. Biotechnol. 11 242-250
  • [3] Sakai S(1998)-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from J. Microb. Biotechnol. 8 710-713
  • [4] Yamamoto I(1998)Purification and properties of cyclodextrin glucanotransferase synthesizing of 2- J. Food Sci. Nutr. 3 225-229
  • [5] Bae KM(1998)- Biol. Pharm. Bull. 21 662-666
  • [6] Kim SK(1984)-D-glucopyranosyl L-ascorbic acid from J. Agric. Food Chem. 32 21-28
  • [7] Kong IS(1969) sp. JB-13 Biochemistry 8 2652-2655
  • [8] Jun HK(1970)Identification of L-ascorbic acid 2- Vitamin 41 387-398
  • [9] Jun HK(1990)- J. Biochem. 107 222-227
  • [10] Bae KM(2001)-glucoside, a stable form of ascorbic acid, in Kimchi Biotechnol. Lett. 23 727-730
1234567