Occurrence of reducing terminal N-acetylglucosamine 3-sulfate and fucosylated outer chains in acidic N-glycans of porcine zona pellucida glycoproteins

被引：0
作者：
Etsuko Mori
Jerry L Hedrick
Nate J Wardrip
Tsuneatsu Mori
Seiichi Takasaki
机构：
[1] University of Tokyo,Department of Immunology, Institute of Medical Science
[2] University of California,The Section of Molecular and Cellular Biology
[3] University of Tokyo,Departments of Biochemistry, Institute of Medical Science
来源：
Glycoconjugate Journal | 1998年 / 15卷
关键词：
acidic N-glycan; zona pellucida; sulfation; fucosylation; ZP, zona pellucida; AAL, Aleuria aurantia lectin; Con A, concanavalin A; aMan, anhydromannose; PVL, Psathyrella velutina lectin; UEA, Ulex europaeus agglutinin; SSA, Sambucus sieboidiana agglutinin; Lea, Lewis a; Lex, Lewis X. Nomenclature for the ZP glycoproteinsused here is adapted from that proposed by Harris et al.[16] and discussed in greater detail by Hedrick, J. L. [47].Terms used for pig ZP glycoproteins in this paper andequivalent terms used in past publications from ourlaboratory and for the mouse ZP glycoproteins are thefollowing: ZPA=90 K (pig), ZP2 (mouse); ZPB=55 Kα (pig); ZPC=55 Kβ (pig), ZP3 (mouse). Subscript OTindicates NaB3H4-reduced glycans.;
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摘要：
Structures of acidic N-glycans released from porcine zona pellucida glycoproteins by hydrazinolysis were studied. The results indicated that the acidic glycans are of mono- to tetraantennary complex-type with and without N-acetyllactosamine repeating units. Sulfated residues are not only located at the C-6 position of GlcNAc included in the N-acetyllactosamine repeating units, but also at the C-6 position of GlcNAc in the non-repeated antennae and at the C-3 position of reducing terminal GlcNAc residue. Analysis of the oligosaccharide fragments released by endo-β-galactosidase digestion and by hydrazine/nitrous acid treatment also revealed that various sulfated and non-sulfated forms of fucosylated structures such as Fucα1→2Galβ1→4(±SO−3→6)GlcNAc (type 2H), Galβ1→4(Fucα1→3)(±SO−3→6)GlcNAc(Lex) and Fucα1→3 or 4(±SO−3→6)GlcNAc, are expressed in the repeated outer chain moieties.
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页码：447 / 456
页数：9
相关论文
共 167 条
[11]  
Litscher ES(1985)undefined J Exp Zool 233 479-83
[12]  
Juntunen K(1989)undefined J Reprod Fert 86 559-65
[13]  
Seppo A(1987)undefined Dev Biol 121 478-88
[14]  
Penttila L(1987)undefined J Biol Chem 262 564-71
[15]  
Niemela R(1994)undefined DNA Sequence 4 361-93
[16]  
Renkonen O(1991)undefined Biochemistry 30 2078-87
[17]  
Wassarman PM(1993)undefined Eur J Biochem 214 763-9
[18]  
Huang TTF(1992)undefined Eur J Biochem 209 883-94
[19]  
Ohzu JE(1994)undefined Eur J Biochem 221 491-512
[20]  
Yanagimachi R(1982)undefined Methods Enzymol 28 702-13
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