Occurrence of reducing terminal N-acetylglucosamine 3-sulfate and fucosylated outer chains in acidic N-glycans of porcine zona pellucida glycoproteins

被引：0
作者：
Etsuko Mori
Jerry L Hedrick
Nate J Wardrip
Tsuneatsu Mori
Seiichi Takasaki
机构：
[1] University of Tokyo,Department of Immunology, Institute of Medical Science
[2] University of California,The Section of Molecular and Cellular Biology
[3] University of Tokyo,Departments of Biochemistry, Institute of Medical Science
来源：
Glycoconjugate Journal | 1998年 / 15卷
关键词：
acidic N-glycan; zona pellucida; sulfation; fucosylation; ZP, zona pellucida; AAL, Aleuria aurantia lectin; Con A, concanavalin A; aMan, anhydromannose; PVL, Psathyrella velutina lectin; UEA, Ulex europaeus agglutinin; SSA, Sambucus sieboidiana agglutinin; Lea, Lewis a; Lex, Lewis X. Nomenclature for the ZP glycoproteinsused here is adapted from that proposed by Harris et al.[16] and discussed in greater detail by Hedrick, J. L. [47].Terms used for pig ZP glycoproteins in this paper andequivalent terms used in past publications from ourlaboratory and for the mouse ZP glycoproteins are thefollowing: ZPA=90 K (pig), ZP2 (mouse); ZPB=55 Kα (pig); ZPC=55 Kβ (pig), ZP3 (mouse). Subscript OTindicates NaB3H4-reduced glycans.;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Structures of acidic N-glycans released from porcine zona pellucida glycoproteins by hydrazinolysis were studied. The results indicated that the acidic glycans are of mono- to tetraantennary complex-type with and without N-acetyllactosamine repeating units. Sulfated residues are not only located at the C-6 position of GlcNAc included in the N-acetyllactosamine repeating units, but also at the C-6 position of GlcNAc in the non-repeated antennae and at the C-3 position of reducing terminal GlcNAc residue. Analysis of the oligosaccharide fragments released by endo-β-galactosidase digestion and by hydrazine/nitrous acid treatment also revealed that various sulfated and non-sulfated forms of fucosylated structures such as Fucα1→2Galβ1→4(±SO−3→6)GlcNAc (type 2H), Galβ1→4(Fucα1→3)(±SO−3→6)GlcNAc(Lex) and Fucα1→3 or 4(±SO−3→6)GlcNAc, are expressed in the repeated outer chain moieties.
引用
收藏
页码：447 / 456
页数：9
相关论文
共 167 条
[1]  
Bleil JD(1986)undefined J Cell Biol 102 1363-71
[2]  
Wassarman PM(1988)undefined Dev Biol 128 376-85
[3]  
Bleil JD(1992)undefined Nature 357 589-93
[4]  
Greve JM(1988)undefined Proc Natl Acad Sci USA 85 6778-82
[5]  
Wassarman PM(1995)undefined Biochemistry 34 4662-9
[6]  
Miller DJ(1982)undefined Gamete Res 5 355-61
[7]  
Macek MB(1987)undefined FEBS Lett 226 38-42
[8]  
Shur BD(1991)undefined Development 111 1165-72
[9]  
Bleil JD(1993)undefined Biochem Biophys Res Commun 196 196-202
[10]  
Wassarman PM(1995)undefined Dev Biol 168 575-83
12345678910