Immobilization of active lipase B from Candida antarctica on the surface of polyhydroxyalkanoate inclusions

被引：0
作者：
Anika C. Jahns
Bernd H. A. Rehm
机构：
[1] Massey University,Institute of Fundamental Sciences
[2] PolyBatics Ltd,Department of Medical Biosciences
[3] BioCommerce Centre,undefined
[4] MacDiarmid Institute for Advanced Materials and Nanotechnology,undefined
[5] Umeå University,undefined
来源：
Biotechnology Letters | 2015年 / 37卷
关键词：
Beads; Lipase; PHA synthase (PhaC); Polyhydroxyalkanoate beads;
D O I：
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中图分类号：
学科分类号：
摘要：
Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli, were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fusion of the PHA synthase, PhaC, to lipase B yielded active PHA lipase beads capable of hydrolyzing glycerol tributyrate. Lipase B beads showed stable activity over several weeks and re-usability without loss of function.
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页码：831 / 835
页数：4
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Jahns AC(undefined)undefined undefined undefined undefined-undefined
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Rehm BHA(undefined)undefined undefined undefined undefined-undefined
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