Immobilization of active lipase B from Candida antarctica on the surface of polyhydroxyalkanoate inclusions

被引：0
作者：
Anika C. Jahns
Bernd H. A. Rehm
机构：
[1] Massey University,Institute of Fundamental Sciences
[2] PolyBatics Ltd,Department of Medical Biosciences
[3] BioCommerce Centre,undefined
[4] MacDiarmid Institute for Advanced Materials and Nanotechnology,undefined
[5] Umeå University,undefined
来源：
Biotechnology Letters | 2015年 / 37卷
关键词：
Beads; Lipase; PHA synthase (PhaC); Polyhydroxyalkanoate beads;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli, were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fusion of the PHA synthase, PhaC, to lipase B yielded active PHA lipase beads capable of hydrolyzing glycerol tributyrate. Lipase B beads showed stable activity over several weeks and re-usability without loss of function.
引用
收藏
页码：831 / 835
页数：4
相关论文
共 49 条
[1]  
Chen B(2008) lipase B chemically immobilized on epoxy-activated micro—and nanobeads: catalysts for polyester synthesis Biomacromolecules 9 463-471
[2]  
Hu J(1969)The effect of sonication on lipase activity Lipids 5 362-365
[3]  
Miller EM(2009)Bacterial polyhydroxyalkanoate granules: biogenesis, structure, and potential use as nano-/micro-beads in biotechnological and biomedical applications Biomacromolecules 10 660-669
[4]  
Xie W(1998)Microbial lipases form versatile tools for biotechnology Trends Biotechnol 16 396-403
[5]  
Cai M(2008)Multifunctional inorganic-binding beads self-assembled inside engineered bacteria Bioconjug Chem 19 2072-2080
[6]  
Gross RA(2007)Improvement of enzyme activity, stability and selectivity via immobilization techniques Enzyme Microb Technol 40 1451-1463
[7]  
Goodman LP(2003)Imaging the distribution and secondary structure of immobilized enzymes using infrared microspectroscopy Biomacromolecules 4 70-74
[8]  
Dugan LR(2001)A novel alkaline lipase from Process Biochem 37 187-192
[9]  
Grage K(2003) for detergent formulation Biochem J 376 15-33
[10]  
Jahns AC(2006)Polyester synthases: natural catalysts for plastics Biotechnol Lett 28 207-213
12345