Artificial endosperm of Cleopatra tangerine zygotic embryos: a model for somatic embryo encapsulation

被引：0
作者：
Nadina Nieves
Jose C. Lorenzo
Maria de los A. Blanco
Justo González
Hipólito Peralta
Martha Hernández
Ramón Santos
Oscar Concepción
Carlos G. Borroto
Eduviges Borroto
Raúl Tapia
Marcos E. Martinez
Zaida Fundora
Alfredo González
机构：
[1] I.S.A.C.A.,Biochemistry Lab., Bioplant Centre
来源：
Plant Cell, Tissue and Organ Culture | 1998年 / 54卷
关键词：
Citrus reshni; conversion; germination; synthetic seed; zeolite;
D O I：
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中图分类号：
学科分类号：
摘要：
Synthetic seed technology may be of value in breeding programs and allow the propagation of many elite genotype-derived plants in a short time. In this work, a range of artificial endosperm treatments of Cleopatra tangerine zygotic embryos were evaluated for suitability for encapsulation of somatic embryos. Different complexing ions in the form of alginate capsules, zeolite as an ion exchanger and the relationship between capsule-nutrient gel on germination of zygotic embryos, were evaluated. Artificial endosperm assays showed that abscisic acid (1 μM) and mannitol (0.25 M) delayed germination and conversion of zygotic embryos, whereas amino acid supplements (proline, glutamic acid and arginine) accelerated the conversion process. An artificial endosperm was used to encapsulate somatic and zygotic embryos. After encapsulation, zygotic embryos germinated after four days of culture while somatic embryos germinated asynchronously after 20 days. Somatic embryo-derived plantlets showed greater vigour than zygotic embryo-derived plantlets. Results showed that this artificial endosperm is adequate for Cleopatra tangerine somatic embryo germination and conversion into plants.
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页码：77 / 83
页数：6
相关论文
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