A fluorometric histidine biosensor based on the use of a quencher-labeled Cu(II)-dependent DNAzyme

被引：0
作者：
Zhuling Chen
Qun He
Mengmeng Zhao
Cuiying Lin
Fang Luo
Zhenyu Lin
Guonan Chen
机构：
[1] Fuzhou University,College of Chemistry
[2] Fuzhou University,School of Law
[3] Fuzhou University,Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety
[4] Fuzhou University,College of Biological Science and Engineering
来源：
Microchimica Acta | 2017年 / 184卷
关键词：
DNAzyme; Cofactors; FAM-labeled DNA; Catalytic beacon; Fluorometry; Human urine samples;
D O I：
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学科分类号：
摘要：
The authors describe a biosensor for histidine that is based on the use of a DNAzyme catalytic beacon. The Cu(II)-dependent DNA-cleaving DNAzyme (Cu-Enzyme) was modified with a quencher (BHQ1) at its 5′ end, and the corresponding substrate strand (Cu-Sub) was modified with a quencher and the FAM fluorophore at its 5′ and 3′ ends, respectively. The green FAM emission of the system is completely quenched after the Cu-Enzyme is hybridized with Cu-Sub. The presence of Cu(II) triggers the cleavage of the Cu-Sub so that fluorescence recovers. Histidine forms a complex with Cu(II) ion. The complex is not capable of cleaving Cu-Sub effectively so that the fluorescence of the system is not restored. These findings were exploited to design a robust and sensitive assay for the determination of histidine. Fluorescence intensity is linearly related to the concentration of histidine in the range between 0.05 and 40 μM, and the detection limit is 20 nM. The method has been successfully applied to the determination of histidine in (spiked) human urine and gave satisfying results.
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页码：4015 / 4020
页数：5
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  • [1] Bessho Y(2014)Characterization of an avian histidine decarboxylase and localization of histaminergic neurons in the chicken brain Neurosci Lett 578 106-110
  • [2] Iwakoshi-Ukena E(1997)Metal-catalyzed oxidation of histidine in human growth hormone mechanism, isotope effects, and inhibition by a mild denaturing alcohol J Biol Chem 272 9019-9029
  • [3] Tachibana T(2005)Histidine-rich glycoprotein: a novel adaptor protein in plasma that modulates the immune, vascular and coagulation systems Immunol Cell Biol 83 106-118
  • [4] Maejima S(2008)A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining Angew Chem 120 3795-3799
  • [5] Taniuchi S(2008)Consequences of low plasma histidine in chronic kidney disease patients: associations with inflammation, oxidative stress, and mortality Am J Clin Nutr 87 1860-1866
  • [6] Masuda K(1975)Low free serum histidine concentration in rheumatoid arthritis. A measure of disease activity J Clin Invest 55 1164-354
  • [7] Shikano K(1993)Serum amino acids, liver status, and antiepileptic drug therapy in epilepsy Epilepsia 34 347-243
  • [8] Kondo K(2006)Determination of tryptophan and histidine by adsorptive cathodic stripping voltammetry using H-point standard addition method Anal Chim Acta 580 236-131
  • [9] Furumitsu M(2009)Iron (III) porphyrin bearing 2, 6-di-tert-butylphenol pendants deposited onto gold electrodes for amperometric determination of l-histidine Talanta 78 126-1081
  • [10] Ukena K(2004)Electrochemical detection of amino acids at carbon nanotube and nickel–carbon nanotube modified electrodes Analyst 129 1076-21
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