Detection of Pepino mosaic virus isolates from tomato by one-step reverse transcription loop-mediated isothermal amplification

被引：0
作者：
Beata Hasiów-Jaroszewska
Natasza Borodynko
机构：
[1] Institute of Plant Protection-National Research Institute,Department of Virology and Bacteriology
来源：
Archives of Virology | 2013年 / 158卷
关键词：
Lamp Assay; Triple Gene Block; Magnesium Pyrophosphate; Tomato Black Ring Virus; Final Total Volume;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique for the amplification of DNA under isothermal conditions. For the first time, we applied this method to develop a simple and sensitive tool for the detection of Pepino mosaic virus (PepMV). PepMV is an emerging pathogen that causes yield and quality losses in tomato crops. Specific RT-LAMP primers for PepMV detection were designed based on triple gene block sequences. The reaction was performed in a single tube at 65 °C for 30 min. The RT-LAMP assay is easy to perform and inexpensive, and it may be applied in the rapid and specific diagnosis of PepMV.
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收藏
页码：2153 / 2156
页数：3
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