Immunolcocalization of actin in intact and DNA- and histone-depleted nuclei and chromosomes of allium cepa

被引：0
作者：
Wan L.H. [1 ]
Xing M. [1 ,2 ]
机构：
[1] Institute of Genetics and Cytology, Northeast Normal University
[2] Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, Jilin Province
来源：
Cell Research | 1998年 / 8卷 / 1期
关键词：
Actin; Allium cepa; Chromosomes; Nuclei;
D O I：
10.1038/cr.1998.6
中图分类号：
学科分类号：
摘要：
The presence of actin in eukaryotic nuclei and chromosomes, and especially in higher plant nuclei and chromosomes, has not been well established. We detected actin in meristematic cells of Allium cepa with indirect immunofluorescence technique and observed bright fluorescence in the intact nuclei and chromosomes, indicating that actin is present in the nuclei and chromosomes of the higher plant. We labeled sections of the meristematic cells of A. cepa with immunogold technique, gold particles were found over the whole nuclei and a number of gold particles were concentrated in condensed chromatin and nucleoli, confirming the results of the immunofluoresence observations. We treated the nuclei and chromosomes of A. cepa with DNase I and 2M NaCl and obtained DNA- and histone-depleted nuclei and chromosomes. Indirect immunofluorescence tests showed that the DNA- and histone-depleted nuclei and chromosomes reacted positively with the anti-actin antibodies. These results demonstrate that actin exists not only in intact nuclei and chromosomes, but also in DNA- and histone-depleted nuclei and chrmosomes of the plant. In addition, our immuno-fluorescence tests indicate that tropomyosin is present in the nuclei and chromosomes of A. cepa.
引用
收藏
页码：51 / 62
页数：11
相关论文
共 36 条
  • [1] Alberts B., Bray D., Lewis J., Raff M., Roberts K., Watson J.D., Molecular Biology of the Cell, 3ndEdition, pp. 821-834, (1994)
  • [2] Jockusch B.M., Becker M., Hindennach L., Jockusch H., Slime mold actin: Homology to vertebrateactin and presence in the nucleus, Exp Cell Res, 89, pp. 241-246, (1974)
  • [3] Douvas A.S., Harrington C.A., Bonner J., Major nonhistone proteins of rat liver chromatin: Preliminary identification of myosin, actin, tubulin and tropomyosin, Proc Nat Acad Sci USA, 72, pp. 3902-3906, (1975)
  • [4] Lestourgeon W.M., Foreer A., Yang Y.Z., Bertram J.S., Rusch H.P., Contractile proteins: Major components of nuclear and chromosome nonhistone proteins, Biochem and Biophy Acta, 379, pp. 529-552, (1975)
  • [5] Lestourgeon W.M., The occurrence of contractile proteins in nuclei and their possible functions, The Cell Nucleus, 6, pp. 305-326, (1978)
  • [6] Peterson J.L., McConkey E.H., Non-histone chromosomal proteins from HeLa cells, Journal OfBiological Chemistry, 251, pp. 548-554, (1976)
  • [7] Clark T.G., Merriam R.W., Diffusible and bound actin in nuclei of Xenopus laevis oocytes, Cell, 12, pp. 883-891, (1977)
  • [8] Clark T.G., Rosenbaum J.L., An actin filament matrix in hand-isolated nuclei of X. laevis oocytes, Cell, 18, pp. 1101-1108, (1979)
  • [9] Pederson T., Isolation and characterization of chromatin from the cellular slime mold Dic-tyostelium discoideum, Biochemistry, 16, pp. 2771-2777, (1977)
  • [10] Farr R.M., Luckow V., Sundharadas G., Major nonhistone proteins of bovine lymphocyte chromatin: Identification of tubulin and actin, Exp Cell Res, 121, pp. 428-430, (1979)
1234