Molecular surveillance of respiratory viruses with bioaerosol sampling in an airport

被引:40
作者
Bailey E.S. [1 ,2 ]
Choi J.Y. [1 ,2 ]
Zemke J. [1 ,2 ]
Yondon M. [1 ,2 ]
Gray G.C. [1 ,2 ,3 ,4 ]
机构
[1] Duke Global Health Institute, Duke University, 310 Trent Drive, Durham, 27710, NC
[2] Division of Infectious Diseases, Duke University School of Medicine, DUMC Box 102359, Durham, 27710, NC
[3] Global Health Research Center, Duke-Kunshan University, No. 8 Duke Avenue, Kunshan, Jiangsu
[4] Emerging Infectious Diseases Program, Duke-NUS Medical School, 8 College Road, Singapore
关键词
Air travel; Bioaerosol; Epidemiology; Respiratory viruses;
D O I
10.1186/s40794-018-0071-7
中图分类号
学科分类号
摘要
Recognizing that crowded, high-traffic airports and airplanes have been implicated in respiratory disease transmission, we partnered with administrators of Raleigh Durham International Airport (RDU) in conducting a pilot study of aerosol surveillance for respiratory viruses at RDU. From January to March 2018 we used NIOSH 2-stage samplers to collect 150 min aerosol samples in crowded areas at RDU. Four (17%) of the 24 samples were positive for known respiratory pathogens including influenza D virus and adenovirus. These results suggest the feasibility of employing bioaerosol surveillance techniques in public transportation areas, such as airports, as a noninvasive way to detect and characterize novel respiratory viruses. © 2018 The Author(s).
引用
收藏
相关论文
共 19 条
[11]  
WHO Information for Moleduclar Diagnosis of Influenza Virus in Humans Update, (2014)
[12]  
Selvaraju S.B., Selvarangan R., Evaluation of three influenza a and B real-time reverse transcription-PCR assays and a new 2009 H1N1 assay for detection of influenza viruses, J Clin Microbiol, 48, 11, pp. 3870-3875, (2010)
[13]  
Pabbaraju K., Et al., Detection of influenza C virus by a real-time RT-PCR assay, Influenza Other Respir Viruses, 7, 6, pp. 954-960, (2013)
[14]  
Oberste M.S., Et al., Characterizing the picornavirus landscape among synanthropic nonhuman primates in Bangladesh, 2007 to 2008, J Virol, 87, 1, pp. 558-571, (2013)
[15]  
Loens K., Et al., Performance of different mono- and multiplex nucleic acid amplification tests on a multipathogen external quality assessment panel, J Clin Microbiol, 50, 3, pp. 977-987, (2012)
[16]  
Bil-Lula I., Et al., Improved real-time PCR assay for detection and quantification of all 54 known types of human adenoviruses in clinical samples, Med Sci Monit, 18, 6, pp. BR221-BR228, (2012)
[17]  
Lu X., Erdman D.D., Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene, Arch Virol, 151, 8, pp. 1587-1602, (2006)
[18]  
Wellehan J.F., Et al., Detection and analysis of six lizard adenoviruses by consensus primer PCR provides further evidence of a reptilian origin for the atadenoviruses, J Virol, 78, 23, pp. 13366-13369, (2004)
[19]  
Scott M.K., Et al., Human adenovirus associated with severe respiratory infection, Oregon, USA, 2013-2014, Emerg Infect Dis, 22, 6, pp. 1044-1051, (2016)