Disposable amperometric biosensor for the determination of tyramine using plasma amino oxidase

被引：1
作者：
Ana Calvo-Pérez
Olga Domínguez-Renedo
María Asunción Alonso-Lomillo
María Julia Arcos-Martínez
机构：
[1] University of Burgos,Department of Chemistry, Faculty of Sciences
来源：
Microchimica Acta | 2013年 / 180卷
关键词：
Biogenic amines; PAO; Biosensor; Screen-printed electrodes; Tyramine;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We have developed screen–printed carbon electrodes for the determination of tyramine (Tyr) via plasma amine oxidase. The enzyme was immobilized on the carbon working electrode by cross–linking it with bovine serum albumin using glutaraldehyde. The employment of the mediator hydroxymethylferrocene lowers the working potential to +260 mV (vs. a screen–printed Ag/AgCl reference electrode). The effects of pH, potential and mediator concentration were optimized and resulted in reproducibility and repeatability values of 8.6 % and 8.7 %, respectively. Response is linear in the range from 2 to 164 μM, and the limit of detection is 2.0 ± 0.18 μM. The effects of potentially interfering biogenic amines such as putrescine, cadaverine, histamine, spermine, spermidine and tryptamine were also evaluated. The biosensor was successfully applied to the determination of Tyr in cheese.
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页码：253 / 259
页数：6
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