Improved GC for Isosorbide-5-mononitrate Determination in Dog Plasma and Its Application in Pharmacokinetics

The existing methods for the extraction and determination of isosorbide-5-mononitrate (5-ISMN) in biological samples are too complicated, poorly reproducible, and time-consuming. Therefore a procedure is described for the extraction of 5-ISMN from plasma with ethyl acetate and saturated potassium carbonate solution. The procedure of extraction is simple, and has a high recovery. At the same time, an improved, simple, rapid, and specific analytical method for the determination of 5-ISMN in dog plasma by capillary gas chromatography with electron-capture detection was developed. O-Dinitrobenzene was used as an internal standard. This method was successfully applied to a pharmacokinetic study on 5-ISMN.