Modulatory effect of resveratrol on SIRT1, SIRT3, SIRT4,PGC1α and NAMPT gene expression profiles in wild-type adult zebrafish liver

被引：0
作者：
Helena Schirmer
Talita Carneiro Brandão Pereira
Eduardo Pacheco Rico
Denis Broock Rosemberg
Carla Denise Bonan
Maurício Reis Bogo
André Arigony Souto
机构：
[1] Pontifícia Universidade Católica do Rio Grande do Sul,Laboratório de Química de Produtos Naturais, Departamento de Química Pura, Faculdade de Química
[2] Universidade Feevale,Laboratório de Biomedicina
[3] Pontifícia Universidade Católica do Rio Grande do Sul,Laboratório de Biologia Genômica e Molecular, Departamento de Biologia Celular e Molecular, Faculdade de Biociências
[4] Universidade Federal do Rio Grande do Sul,Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde
[5] Pontifícia Universidade Católica do Rio Grande do Sul,Laboratório de Neuroquímica e Psicofarmacologia, Departamento de Biologia Celular e Molecular, Faculdade de Biociências
来源：
Molecular Biology Reports | 2012年 / 39卷
关键词：
Resveratrol; Sirtuins; Pgc1 alpha; NAMPT; Liver;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Sirtuins (SIRTs) are NAD+-dependent deacetylases that catalyze the hydrolysis of acetyl-lysine residues. They play an important role in many physiological and pathophysiological processes, such as the regulation of lifespan and the prevention of metabolic diseases. In this study, we analyzed the effect of resveratrol on the gene expression levels of SIRT1, SIRT3, SIRT4, PGC1α, and NAMPT, as well as its effect on NAD+ and NADH levels, in the liver of non stressed or non impaired wild-type zebrafish. Semiquantative RT-PCR assays showed that resveratrol did not change the mRNA levels of SIRT1 and PGC1α but decreased the expression levels of the SIRT3, SIRT4, and NAMPT genes. The decrease in NAMPT mRNA levels was accompanied by an increase in NADH levels, thereby decreasing the NAD+/H ratio. Taken together, our results suggest that resveratrol plays a modulatory role in the transcription of the NAMPT, SIRT3, and SIRT4 genes. Zebrafish is an interesting tool that can be used to understand the mechanisms of SIRTs and NAMPT metabolism and to help develop therapeutic compounds. However, further investigations using healthy experimental animals are required to study the modulation of the SIRT and NAMPT genes by resveratrol before it is used as a nutraceutical compound in healthy humans.
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页码：3281 / 3289
页数：8
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