Evaluation of the E test for fluconazole susceptibility testing ofCandida albicans isolates from oropharyngeal candidiasis

被引：0
作者：
E. Dannaoui
S. Colin
J. Pichot
M. A. Piens
机构：
[1] Université Claude Bernard Lyon I,Laboratoire de Parasitologie et Mycologie Médicale
来源：
European Journal of Clinical Microbiology and Infectious Diseases | 1997年 / 16卷
关键词：
Minimum Inhibitory Concentration; Fluconazole; Candida Albicans; Itraconazole; Candidiasis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The aim of the present study was to evaluate the utility of the E test in determining the antifungal susceptibility ofCandida albicans. Reproducibility of the E test was determined for amphotericin B, fluconazole, and itraconazole using three different solid media: RPMI 1640, Casitone, and yeast nitrogen base agar. Minimum inhibitory concentrations (MICs) were comparable (results at ±2 dilutions) in 92% of the tests for amphotericin B and in 100% for fluconazole and itraconazole. Determination of MIC endpoints was easiest on Casitone agar.Candida albicans isolates from 23 patients undergoing fluconazole therapy for oropharyngeal candidiasis were tested for fluconazole susceptibility. Good correlation was obtained between the MICs of fluconazole and clinical outcome. Clinical failure was associated with strains for which MICs were ≥48 Μg/ml. These results suggest that the E test has potential utility for fluconazole susceptibility testing of clinical yeast isolates.
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页码：228 / 232
页数：4
相关论文
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  • [1] Espinel-Ingroff A(1991)Comparison study of broth macrodilution and microdilution antifungal susceptibility tests Journal of Clinical Microbiology 29 1089-1094
  • [2] Kerkering TM(1992)E test, an antimicrobial susceptibility testing method with broad clinical and epidemiologic application Antimicrobic Newsletter 8 1-8
  • [3] Goldson PR(1994)E test for antifungal susceptibility testing of yeasts Diagnostic Microbiology and Infectious Disease 19 217-220
  • [4] Shadomy S(1994)Comparison of broth macrodilution, broth microdilution, and E test antifungal susceptibility tests for fluconazole Journal of Clinical Microbiology 32 2099-2102
  • [5] Sanchez ML(1995)Comparison of E test and National Committee for Clinical Laboratory Standards broth macrodilution method for azole antifungal susceptibility testing Journal of Clinical Microbiology 33 535-540
  • [6] Jones RN(1995)Comparison of E test and National Committee for Clinical Laboratory Standards broth macrodilution method for antifungal susceptibility testing: enhanced ability to detect amphotericin B-resistant Antimicrobial Agents and Chemotherapy 39 2520-2522
  • [7] Espinel-Ingroff A(1996) isolates Journal of Clinical Microbiology 34 848-852
  • [8] Sewell DL(1996)Interlaboratory evaluation of E test method for testing antifungal susceptibilities of pathogenic yeasts to five antifungal agents by using Casitone agar and solidified RPMI 1640 medium with 2% glucose Journal of Clinical Microbiology 34 1691-1693
  • [9] Pfaller MA(1996)Multisite reproducibility of the E test MIC method for antifungal susceptibility testing of yeast isolates Journal of Clinical Microbiology 34 842-847
  • [10] Barry AL(1995)Fluconazole and amphotericin B antifungal susceptibility testing by National Committee for Clinical Laboratory Standards broth macrodilution method compared with E test and semiautomated broth microdilution test Antimicrobial Agents and Chemotherapy 39 1-8
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