Hydrophilic interaction liquid chromatography (HILIC) in proteomics

被引：0
作者：
Paul J. Boersema
Shabaz Mohammed
Albert J. R. Heck
机构：
[1] Utrecht University,Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences
来源：
Analytical and Bioanalytical Chemistry | 2008年 / 391卷
关键词：
HILIC; Proteomics; Post-translational modification; Two-dimensional liquid chromatography;
D O I：
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学科分类号：
摘要：
In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatography (SCX) and reversed-phase (RP) chromatography is the most widely used combination for the separation of peptides. Here, we review the potential of hydrophilic interaction liquid chromatography (HILIC) as a separation tool in the multidimensional separation of peptides in proteomics applications. Recent work has revealed that HILIC may provide an excellent alternative to SCX, possessing several advantages in the area of separation power and targeted analysis of protein post-translational modifications.
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页码：151 / 159
页数：8
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