The Rep proteins encoded by alphasatellites restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana

被引：26

作者：

Abbas Q. ^{[1
,2
]}

Amin I. ^{[1
]}

Mansoor S. ^{[1
]}

Shafiq M. ^{[1
,2
,3
]}

Wassenegger M. ^{[2
]}

Briddon R.W. ^{[1
]}

机构：

[1] National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad

[2] RLP-Agroscience, AlPlanta - Institute for Plant Research, Neustadt

[3] Department of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat

来源：

VirusDisease | 2019年 / 30卷 / 1期

关键词：

Alphasatellite; Geminivirus; RNA interference; Transcriptional gene silencing;

D O I：

10.1007/s13337-017-0413-5

中图分类号：

学科分类号：

摘要：

Alphasatellites are non-essential satellite-like components associated with geminiviruses. The precise selective advantage to a geminivirus infection of an alphasatellite remains unclear. The ability of the cotton leaf curl Multan alphasatellite (CLCuMuA)-encoded replication-associated protein (Rep) to suppress TGS was investigated by using Nicotiana benthamiana line 16-TGS (16-TGS) harbouring a transcriptionally silenced green fluorescent protein (GFP) transgene. Inoculation of 16-TGS plants with a recombinant Potato virus X (PVX) vector carrying CLCuMuA Rep resulted in restoration of GFP expression. Northern blot analysis confirmed that the observed GFP fluorescence was associated with GFP mRNA accumulation. Inoculation with PVX vectors harbouring a further six Rep proteins, encoded by genetically distinct alphasatellites, were similarly shown to result in 16-TGS plants with restored GFP expression. These results indicate that the alphasatellite-encoded Rep can restore the expression of a transcriptionally silenced GFP transgene in N. benthamiana, indicating that alphasatellites are involved in overcoming host defence. © 2017, Indian Virological Society.

引用

页码：101 / 105

页数：4

共 26 条

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