Isolation and characterization of 13 microsatellite loci in Rhinolophus pusillus (least horseshoe bat) with cross-amplification in five related species

被引：0
作者：
Panyu Hua
Tingting Guo
Wenchao Liu
Shuyi Zhang
Stephen J. Rossiter
机构：
[1] East China Normal University,School of Life Science
[2] University of London,School of Biological and Chemical Sciences, Queen Mary
来源：
Conservation Genetics | 2009年 / 10卷
关键词：
Microsatellite loci; Genetic structure;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We used the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the least horseshoe bat, Rhinolophus pusillus. Seventeen loci were obtained and tested on 31 individuals sampled from Guangxi Province in southern China. Thirteen of these markers were polymorphic with expected heterozygosity ranging from 0.821 to 0.909. A total of 164 alleles were detected and the number of alleles per locus ranged from 9 to 16 (mean 12.6). These polymorphic markers will be used to assess population structure in R. pusillus. In addition, successful cross-amplification in five congeneric bat species suggests most of these markers will also be useful for studying related species.
引用
收藏
页码：597 / 600
页数：3
相关论文
共 23 条
  • [1] Dawson DA(2004)Microsatellite loci for the greater horseshoe bat, Mol Ecol Notes 4 96-100
  • [2] Rossiter SJ(1995) (Rhinolophidae, Chiroptera) and their cross-utility in 17 other bat species J Hered 86 485-486
  • [3] Jones G(2007)FSTAT (Version 1.2): a computer program to calculate F-statistics Mol Ecol Notes 7 486-488
  • [4] Goudet J(1994)Isolation and characterization of microsatellite loci in the flat-headed bat ( Proc Natl Acad Sci U S A 91 88-92
  • [5] Hua PY(2006)) J Mammal 87 1234-1240
  • [6] Chen JP(2005)Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-specific markers Mol Ecol Notes 5 941-944
  • [7] Zhang LB(1989)Phylogenetics of small horseshoe bats from East Asia based on mitochondrial DNA sequence variation Evolution 43 223-225
  • [8] Kandpal RP(1999)Characterization of 14 polymorphic microsatellite loci for the lesser horseshoe bat, Mol Ecol 8 1959-1960
  • [9] Kandpal G(2004) (Rhinolophidae, Chiroptera) Mol Ecol Notes 4 535-538
  • [10] Weissman SM(undefined)Analyzing tables of statistical tests undefined undefined undefined-undefined
123