Mass-Spectrometric Analysis of Proteome of Microvesicles Produced by NK-92 Natural Killer Cells

被引：0
作者：
A. V. Korenevskii
Yu. P. Milyutina
A. A. Zhdanova
K. M. Pyatygina
D. I. Sokolov
S. A. Sel’kov
机构：
[1] D. O. Ott Research Institute of Obstetrics,
[2] Gynecology,undefined
[3] and Reproductology,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2018年 / 165卷
关键词：
mass spectrometry; proteomic analysis; natural killer cells; microvesicles; granzymes;
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学科分类号：
摘要：
Membrane extracellular microvesicles serve as carriers of a wide range of molecules, the most important among these are proteins, lipids, and nucleic acids. Cytotoxic proteins of natural killer cells play a key role in the realization of their cytolytic functions. An important stage in understanding of the distant communication of cells and mechanisms of its regulation is analysis of the proteome composition of microvesicles. We studied the proteomic composition of microvesicles produced by NK-92 natural killer cells. Granzyme A, a specific protein of cytotoxic cells, has been identified in the microvesicles by QTOF-mass spectrometry. It was shown that heat shock proteins, components of the ubiquitin—proteasome system, enzymes of protein biosynthesis and energy metabolism, nuclear and serum proteins, as well as cytoskeleton proteins are associated with the microvesicles.
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页码：564 / 571
页数：7
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  • [1] Govorun VM(2011)Proteomics and peptidomics in fundamental and applied medical studies Rus. J. Bioorgan. Chem. 37 176-190
  • [2] Ivanov VT(2000)The human antimicrobial and chemotactic peptides LL-37 and alpha-defensins are expressed by specific lymphocyte and monocyte populations Blood. 96 3086-3093
  • [3] Agerberth B(2018)Exosomes or microvesicles? Two kinds of extracellular vesicles with different routes to modify protozoan-host cell interaction Theriogenology. 114 173-179
  • [4] Charo J(2014)Proteomic analysis of human placental syncytiotrophoblast microvesicles in preeclampsia Clin. Proteomics. 11 40-289
  • [5] Werr J(2014)Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles Annu. Rev. Cell Dev. Biol. 30 255-1521
  • [6] Olsson B(2005)The platelet microparticle proteome J. Proteome Res. 4 1516-658
  • [7] Idali F(1994)Characterization of a human cell line (NK-92) with phenotypical and functional characteristics of activated natural killer cells Leukemia. 8 652-250
  • [8] Lindbom L(2016)Focus on extracellular vesicles: introducing the next small big thing Int. J. Mol. Sci. 17 170-E977
  • [9] Kiessling R(2007)Granzyme B leakage-induced apoptosis is a crucial mechanism of cell death in nasal-type NK/T-cell lymphoma Lab. Invest. 87 241-198
  • [10] Jörnvall H(2016)Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes Proc. Natl Acad. Sci USA. 113 E968-804
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