Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus

被引:0
作者
Byoung-Shik Shim
Jung-ah Choi
Ho-Hyun Song
Sung-Moo Park
In Su Cheon
Ji-Eun Jang
Sun Je Woo
Chung Hwan Cho
Min-Suk Song
Hyemi Kim
Kyung Joo Song
Jae Myun Lee
Suhng Wook Kim
Dae Sub Song
Young Ki Choi
Jae-Ouk Kim
Huan Huu Nguyen
Dong Wook Kim
Young Yil Bahk
Cheol-Heui Yun
Man Ki Song
机构
[1] International Vaccine Institute,Laboratory Science Division
[2] Seoul National University,Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, and the Center for Agricultural Biomaterials, and Center for Food Safety and Toxicology
[3] Seoul National University,Department of Oral Microbiology and Immunology, Dental Research Institute, and BK21 Program, School of Dentistry
[4] Chungbuk National University,College of Medicine and Medical Research Institute
[5] Yonsei University College of Medicine,Brain Korea 21 Project for Medical Sciences
[6] Korea University,Department of Biomedical Science, College of Health Sciences
[7] Korea Research Institute of Bioscience and Biotechnology,Viral Infectious Disease Research Center
[8] Hanyang University,Department of Pharmacy, College of Pharmacy
[9] Konkuk University,Department of Biotechnology
来源
Journal of Microbiology | 2013年 / 51卷
关键词
Hemagglutinin; mucosal immune response; non-glycosylation; pandemic; sublingual;
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学科分类号
摘要
Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s.l.) route. We found that s.l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i.m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s.l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.
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页码:130 / 135
页数:5
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