Peculiarities of Cell Seeding on Polylactic Acid-Based Scaffolds Fabricated Using Electrospinning and Solution Blow Spinning Technologies

被引：0
作者：
S. A. Afanasiev
E. F. Muslimova
Yu. A. Nashchekina
P. O. Nikonov
Yu. V. Rogovskaya
E. N. Bolbasov
S. I. Tverdokhlebov
机构：
[1] Russian Academy of Sciences,Research Institute of Cardiology, Tomsk National Research Medical Center
[2] Russian Academy of Sciences,Institute of Cytology
[3] National Research Tomsk Polytechnical University,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2017年 / 164卷
关键词：
synthetic scaffold; polylactic acid; electrospinning; solution blow spinning; cell culture;
D O I：
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中图分类号：
学科分类号：
摘要：
We studied the possibility of seeding bone marrow-derived stromal cells onto polylactic acid-based scaffolds fabricated by electrospinning and solution blow spinning technologies. The cells were applied to the scaffolds by dynamic seeding and scaffolds were then cultured in Petri dishes in culture medium for 3 days. Cell migration to the Petri dish surface was noted only for scaffolds fabricated by electrospinning technology, but DAPI staining confirmed the presence of cells in both scaffolds. The mean number of cells in scaffolds fabricated by electrospinning and solution blow spinning was 56±9 and 81±6, respectively. The scaffold fabricated by solution blow spinning was more effectively (p<0.05) colonized by cells due to its more optimal spatial structure.
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页码：281 / 284
页数：3
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