Detection and molecular characterization of a novel piscine-myocarditis-like virus from baitfish in the USA

被引：0
作者：
Sunil K. Mor
Nicholas B. D. Phelps
机构：
[1] Minnesota Veterinary Diagnostic Laboratory,Department of Veterinary Population Medicine
[2] University of Minnesota,undefined
来源：
Archives of Virology | 2016年 / 161卷
关键词：
Reverse Transcription Polymerase Chain Reaction; Atlantic Salmon; Retail Outlet; Viral Hemorrhagic Septicemia Virus; Reverse Transcription Polymerase Chain Reaction Assay;
D O I：
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中图分类号：
学科分类号：
摘要：
During a survey of apparently healthy baitfish from the state of Minnesota, a novel piscine-myocarditis-like virus (PMCLV) was detected in golden shiners (Notemigonus crysoleucas). The nearly complete genome sequence is 5819 nt long, including a partial 5’ untranslated region (UTR) of 100 nt. The sequence is divided into three ORFs: the complete ORF1 and ORF2, encoding proteins of 818 and 831 amino acids, respectively, and a partial ORF3 encoding 248 amino acids of the corresponding protein. This novel virus sequence was most closely related to piscine myocarditis virus (PMCV), with a 49.0 % and 58.2 % amino acid identity in the ORF1 (YP005481249)- and ORF2 (YP004581250)-encoded proteins, respectively. Six of 56 retail outlets (e.g., bait shops) were positive during the 2014–2015 survey, indicating a 10.7 % prevalence of the novel virus in this population of golden shiners. Currently, there is no disease that is known to be associated with this virus.
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页码：1925 / 1931
页数：6
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