Lipoplexes of dicationic gemini surfactants with DNA: Structural features of DNA compaction and transfection efficiency

被引：0
作者：
D. A. Faizullin
Yu. F. Zuev
L. Ya. Zakharova
A. G. Pokrovsky
V. A. Korobeinikov
T. A. Mukhametzyanov
A. I. Konovalov
机构：
[1] Kazan Scientific Center of the Russian Academy of Sciences,Kazan Institute of Biochemistry and Biophysics
[2] Russian Academy of Sciences,Arbuzov Institute of Organic and Physical Chemistry, Kazan Research Center
[3] Novosibirsk State University,undefined
[4] Kazan (Volga Region) Federal University,undefined
来源：
Doklady Biochemistry and Biophysics | 2015年 / 465卷
关键词：
Transfection Efficiency; Circular Dichroism Spectrum; HEK293T Cell; Gemini Surfac; DOKLADY Biochemistry;
D O I：
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中图分类号：
学科分类号：
摘要：
The internal structure of DNA lipoplexes with hydroxyethylated alkylammonium gemini surfactants (GS) with high transfection activity was studied by circular dichroism. It was shown that the efficiency of transfection of HEK293T cells with the pEGFP-N1 circular plasmid was different from zero only in the region of existence of chiral supramolecular DNA-GS complexes and reaches a maximum at concentrations at which the spontaneous aggregation of components is observed.
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页码：432 / 435
页数：3
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