The genotypes of citrus tristeza virus isolates from China revealed by sequence analysis of multiple molecular markers

被引：0
|
作者：
Guan-wei Wu
Song Pan
Guo-ping Wang
Min Tang
Yong Liu
Fan Yang
Ni Hong
机构：
[1] Huazhong Agricultural University,National Key Laboratory of Agromicrobiology
[2] Huazhong Agricultural University,The Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology
来源：
Archives of Virology | 2013年 / 158卷
关键词：
Citrus Tristeza Virus; Marker Sequence; Reference Isolate; Citrus Tristeza Virus Isolate; B165 Marker;
D O I：
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中图分类号：
学科分类号：
摘要：
The genotypes of ten citrus tristeza virus (CTV) isolates from central China were determined by examining multiple molecular markers (MMMs) using 11 primer pairs. The results revealed that one isolate contained a single T30 genotype, two isolates contained a single VT genotype, and the other seven isolates were mixtures of two or more genotypes. Sequence analysis of amplified MMMs showed a high genetic diversity in Chinese CTV populations. The genotypes resembling T36, RB and B165 were identified from Chinese CTV isolates for the first time. Our results suggest that genotype assignment of CTV cannot be based solely on the amplification profiles of MMMs, and sequencing of MMMs is required.
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页码：231 / 235
页数：4
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