RNA/DNA co-analysis on aged bloodstains from adhesive tapes used for gunshot residue collection from hands

被引：0
作者：
Melanie Grabmüller
Christian Schyma
Burkhard Madea
Tim Eichhorst
Cornelius Courts
机构：
[1] University of Bonn,Institute of Legal Medicine
[2] University of Bern,Institute of Forensic Medicine
[3] University of Schleswig-Holstein Kiel,Institute of Legal Medicine
来源：
Forensic Science, Medicine, and Pathology | 2017年 / 13卷
关键词：
Adhesive tapes; Backspatter; Gunshot residues; Blood specific expression; RNA/DNA co-analysis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In cases of firearm related fatalities a systematic investigation at the scene of death is indispensable to differentiate between self-inflicted and homicidal gunshot injuries. A common method to preserve gunshot residues (GSR) is their collection using adhesive tapes. However, the biological material gathered at the same time by the tapes would be of special interest if backspatter, ejected from the entrance wound against the direction of fire, could be detected. In the present study we examined the success rate of co-analysis of RNA and DNA recovered from biological traces sampled with adhesive tapes. The material originated from eight cases of fatal gunshots, taken from the hands of suspects or victims, examined 5 to 19 years ago for GSR. For all types of adhesive tapes tested, quantity and quality of the co-extracted nucleic acids was insufficient for successful DNA profiling, but was sufficient for the detection of blood-specific micro RNA (miRNA). In summary, sampling trace evidence from the hands of persons involved in fatal gunshots with adhesive tapes has a long-term detrimental effect on biological traces.
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页码：161 / 169
页数：8
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