In vitro inhibitory effect of crab shell extract on human umbilical vein endothelial cell

被引：0
作者：
Pegah Mirzapur
Zahra Rashidi
Leila Rezakhani
Mozafar Khazaei
机构：
[1] Kermanshah University of Medical Sciences,Fertility and Infertility Research Center
来源：
In Vitro Cellular & Developmental Biology - Animal | 2015年 / 51卷
关键词：
Angiogenesis; Cell viability; Crab shell; HUVECs;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Angiogenesis plays an important role in the tissue repair, rheumatoid arthritis, retinopathies, and growth and metastasis of cancer. Endothelial cell proliferation has been introduced as a model of angiogenesis. Crab sell was suggested for cancer treatment in traditional medicine. The aim of the present study was to investigate the in vitro effect of crab shell hydroethanolic extract on the human umbilical vein endothelial cell (HUVEC) proliferation. Crab sell extract was prepared and the effect of its various concentrations (0, 100, 200, 400, 800, 1000 μg/ml) on HUVECs were surveyed in three periods of 24, 48 and 72 h. HUVEC viability, Nitric oxide (NO) secretion and apoptosis were assessed by MTT, Griess and Tunnel methods, respectively. Data were compared by one-way ANOVA. In this study we found HUVECs viability was reduced in dose and time depended manner significantly and HUVECs NO production decreased significantly, too. Apoptosis index was increased significantly. These findings reveal that high concentrations of crab shell extract have anti-proliferative effects on HUVECs and can be used for cancer treatment.
引用
收藏
页码：36 / 41
页数：5
相关论文
共 87 条
[1]  
Auerbach R(2003)Angiogenesis assays: a critical overview Clin Chem 49 32-40
[2]  
Lewis R(2007)Differential involvement of reactive oxygen species in apoptosis caused by the inhibition of protein phosphatase 2A in Jurkat and CCRF-CEM human T-leukemia cells Exp Mol Pathol 83 347-356
[3]  
Shinners B(1998)Chemopreventive agents: selenium Pharmacol Ther 79 179-192
[4]  
Kubai L(2003)NO and angiogenesis Atheroscler Suppl 4 53-60
[5]  
Akhtar N(2000)Endothelial cell apoptosis in angiogenesis and vessel regression Circ Res 87 434-439
[6]  
Boudreau RT(2004)Chitin and chitosan: Chemistry, properties and applications J Sci Ind Res 63 20-31
[7]  
Conrad DM(2009)Regulation of angiogenesis by oxygen and metabolism Dev Cell 16 167-179
[8]  
Hoskin DW(2005)Depolymerized products of chitosan as potent inhibitors of tumor-induced angiogenesis Biochim Biophys Acta 1722 22-29
[9]  
Combs GF(1998)VEGF upregulates ecNOS message, protein, and NO production in human endothelial cells Am J Physiol 274 H1054-1058
[10]  
Gray WP(2001)Methylselenocysteine modulates proliferation and apoptosis biomarkers in premalignant lesions of the rat mammary gland Anticancer Res 21 863-867
123456789