Localization of Telomere-Binding Protein TRF2 in Spermatogenic Cells of Hibernating Frogs Rana temporaria

被引：0
作者：
Travina A.O. [1 ]
Shvets P.K. [2 ]
Pochukalina G.N. [1 ]
Podgornaya O.I. [1 ,3 ]
机构：
[1] Institute of Cytology, Russian Academy of Sciences, St. Petersburg
[2] St. Petersburg State Institute of Technology, St. Petersburg
[3] Department of Cytology and Histology, St. Petersburg State University, St. Petersburg
来源：
Cell and Tissue Biology | 2023年 / 17卷 / 1期
基金：
俄罗斯基础研究基金会; 俄罗斯科学基金会;
关键词：
acrosome; nuage; Rana temporaria; spermatogenesis; TRF2;
D O I：
10.1134/S1990519X2301008X
中图分类号：
学科分类号：
摘要：
Abstract: Telomeric DNA is associated with a protein complex, which is called “shelterin.” There are data indicating that the functions of the shelterin complex are wider than just protecting chromosome ends. There are examples of unusual distribution of the shelterin component, telomere-binding TRF2 protein, in mouse and common frog Rana temporaria oocytes and mouse spermatozoa. In this work, the distribution of TRF2 in the cells of spermatogenic row of the common frog was studied. To confirm the specificity of antibodies to the grass frog TRF2, Western-blot analysis of testis preparations was used. Antibodies selected by immunoblotting were used to determine the localization of TRF2 in spermatogonia and spermatozoa using immunoelectron microscopy. It was demonstrated that TRF2 in spermatogonia is evenly distributed throughout the nucleus. The presence of TRF2 was detected in the cytoplasm of spermatogonia in electron-dense structures. In spermatozoa, some of the TRF2 is located in the acrosome, proving the universality of the phenomenon for vertebrates. © 2023, Pleiades Publishing, Ltd.
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页码：40 / 47
页数：7
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