Isolation and characterization of microsatellite loci in Astroniumgraveolens (Anacardiaceae) and cross amplification in related species

被引：0
作者：
Karman F. Sanchez-Gomez
E. Jacob Cristóbal-Pérez
Nick Harvey
Mauricio Quesada
机构：
[1] Universidad Nacional Autónoma de México,Instituto de Investigaciones en Ecosistemas y Sustentabilidad
[2] Universidad Nacional Autónoma de México,Laboratorio Nacional de Análisis y Síntesis Ecológica (LANASE), Escuela Nacional de Estudios Superiores Unidad Morelia
[3] Genetic Marker Services,undefined
来源：
Molecular Biology Reports | 2020年 / 47卷
关键词：
Anacardiaceae; Dioecy; Habitat fragmentation; Microsatellites;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Microsatellite markers are a useful genetic tool to answer ecological and conservation genetics questions. Microsatellite primers were developed and characterized to evaluate forest fragmentation effects on genetic structure, diversity and gene flow patterns in the dioecious tropical tree Astroniumgraveolens (Anacardiaceae). Using genomic library enrichment, sixteen microsatellite loci were developed for A.graveolens. Polymorphism was evaluated in 80 individuals from four localities in Mexico. The overall number of alleles detected was 63, average alleles per locus 3.9 with a range from one to 11 per locus. Cross amplification trails on related species in the Anacardiaceae family: Spondiaspurpurea and Amphipterygiumadstringens, achieved successful amplification for all primers. Microsatellite markers described here are the first to be characterized for A.graveolens. These genetic markers will be a useful tool to assess the genetic consequences of habitat fragmentation and selective logging on A.graveolens populations. Cross amplification success on S.purpureaandA.adstringens suggest that they may be used for population genetic studies in other species within the family.
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页码：4003 / 4007
页数：4
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