Electrochemistry and biosensing of glucose oxidase immobilized on Pt-dispersed mesoporous carbon

被引：2
|
作者：
Chunping You
Xiang Li
Song Zhang
Jilie Kong
Dongyuan Zhao
Baohong Liu
机构：
[1] Fudan University,Department of Chemistry, Shanghai Key Lab of Molecular Catalysis and Novel Materials, and Institutes of Advanced Materials and Biomedical Sciences
[2] Technology Center,undefined
[3] Bright Dairy and Food Co Ltd,undefined
来源：
Microchimica Acta | 2009年 / 167卷
关键词：
Glucose oxidase; Biosensor; Electron transfer; Mesoporous carbon; Nanoparticles;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A method for immobilizing proteins in a carbon mesoporous material (CMM) containing platinum nanoparticles (Pt-NPs) is demonstrated. Compared to pure CMM or carbon nanotubes, CMM containing Pt-NPs enhances the electron transfer and redox properties of redox enzymes, such as glucose oxidase (GOx), due to a cooperative effect of Pt-NPs and CMM. The quasi-reversible electron transfer of GOx in this system is probed, and the apparent heterogeneous electron transfer rate constants are found to be 66% larger than in pure CMM. The GOx/Pt-CMM based glucose biosensor enables the determination of glucose at a potential of 600 mV (vs. SCE). Its detection limit is 10 times lower, and the sensitivity is 16 times higher than that of the respective biosensor without Pt-NPs.
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