Inverse correlation between salt tolerance and host-adaptation in mycobacteria

被引：9

作者：

Asmar S. ^{[1
]}

Sassi M. ^{[2
]}

Phelippeau M. ^{[1
]}

Drancourt M. ^{[1
]}

机构：

[1] Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, CNRS, UMR 7278, IRD 198, Faculté de Médecine, Aix-Marseille Université, 27, Boulevard Jean Moulin, Marseille Cedex 5

[2] Université de Rennes 1, Rennes

来源：

BMC Research Notes | / 9卷 / 1期

关键词：

Culture; Mycobacterium; Mycobacterium canettii; Mycobacterium tuberculosis; Salt;

D O I：

10.1186/s13104-016-2054-y

中图分类号：

学科分类号：

摘要：

Background: The genus Mycobacterium includes host-adapted organisms regarded as obligate and opportunistic pathogens and environmental organisms. Factors contributing to this wide range of adaptations are poorly known. Results: We studied the salt tolerance of 46 Mycobacterium species of medical interest. Representative strains of the Mycobacterium tuberculosis complex, Mycobacterium avium complex, Mycobacterium chelonae-abscessus complex, Mycobacterium ulcerans, Mycobacterium marinum, Mycobacterium lentiflavum, Mycobacterium fortuitum and Mycobacterium conceptionense were inoculated on Middlebrook 7H10 medium supplemented with 0-10 % sodium chloride. Colonies were counted after 2-4 week incubation at the appropriate 30-37°C temperature depending on the tested strain. Further comparative genomics was done on 15 Mycobacterium strains representing the spectrum of salt-tolerance of mycobacteria. Based on the results the different species were grouped according to their salt tolerance into a "salt-sensitive" group (growth up to ≤3 % salt) containing the M. tuberculosis complex, Mycobacterium chelonae, Mycobacterium lentiflavum, Mycobacterium ulcerans and Mycobacterium marinum; a "salt-intermediate" group (growth between 4 and 6 % salt) comprising Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium chimaera and a "salt-resistant" group (growth up to >6 %) comprising Mycobacterium homonissuis, Mycobacterium bolettii, Mycobacterium fortuitum and Mycobacterium conceptionense. Genomic analysis revealed that 290 genes were unique to species belonging to the salt-sensitive group; and that 15 % were annotated as being functionally associated with the ESX secretion systems Pro-Glu and Pro-Pro-Glu family proteins. Conclusions: In this work we found an inverse correlation between salt tolerance and host adaptation. We thus propose that salinity is one of the multiple factors determining the ecological niches of mycobacteria. © 2016 Asmar et al.

引用

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